研究生: |
陳俊安 Jun-An Chen |
---|---|
論文名稱: |
以聚醚胺改質雙馬來醯亞胺/巴比妥酸 藥物傳遞之應用 N,N’-Bismaleimide-4,4-Diphenylmethane / Barbituric Acid modified by Jeffamine for Drug Delivery |
指導教授: |
陳崇賢
Chorng-Shyan Chern |
口試委員: |
鄭智嘉
Chih-Chia Cheng 邱信程 Hsin-Cheng Chiu 許榮木 Jung-Mu Hsu |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2018 |
畢業學年度: | 106 |
語文別: | 中文 |
論文頁數: | 103 |
中文關鍵詞: | 聚醚胺 、巴比妥酸 、雙馬來醯亞胺 、藥物傳遞 |
外文關鍵詞: | N,N’-Bismaleimide-4,4-Diphenylmethane, Barbituric Acid, Jeffamine, Drug Delivery |
相關次數: | 點閱:271 下載:0 |
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本研究以雙馬來醯亞胺(N,N’-bismaleimide-4,4’-diphenylmethane, BMI) 與巴比妥酸 (Barbituric acid, BTA) 調整BMI/BTA莫爾比為2/1及3/1進行聚合反應,形成STOBA 溶液,再使用Jeffamine M-1000進行改質,分別添加20%JF、30%JF及50%JF,最後形成親水性之STOBA-JF樣品。
因本研究所合成之STOBA-JF具有酸鹼應答性,其在低pH值時, BTA提供的負電荷會愈少,粒徑上升;在高pH值時,JF的立體障礙使粒徑維持不變,故樣品包覆DOX後於低pH值時會因靜電作用力的減弱,而能釋放較多比例之DOX藥物。
而使用HeLa細胞進行細胞毒性測試中所有未包覆DOX之樣品皆無明顯之細胞毒性;但若將樣品包覆DOX則在高濃度下有明顯的細胞毒性,證明具有毒殺癌細胞的功用。
另外於細胞吞噬實驗則能進一步了解包覆DOX之樣品因靜電作用力而吸附於細胞周圍,逐漸被細胞吞噬,隨共同培養之時間增加,細胞數明顯減少,因此此一具有酸鹼應答特性之STOBA-JF樣品有極大的潛力應用於藥物載體。
This work studied the polymerization reaction of N,N-bismaleimide-4,4-diphenylmethane (BMI) with Barbituric acid (BTA). The fisrt step was polymerized to STOBA solution by different BMI/BTA molar ratio. Second, using different proportions of Jeffamine M-1000 to modify STOBA solution so that the STOBA-JF became hydrophilic structure.
At low pH value, BTA would provide less negative charges so that the particles size would increase;at high pH value, the particles size remain the same due to Jeffamine provided steric stabilization. Because of the electrostatic force reduce, STOBA-JF loaded DOX would release more at low pH value.
Since all samples were non cytotoxicity in the HeLa cell cytotoxic experiment;however, all loaded DOX samples have obviously cytotoxicity in high concentration. Furthermore, the cell uptake experiment also told us that the loaded DOX samples would absorb in cell surface and be uptake slowly. As the time of co-cultivation increased, the cell density decrease significantly. As a result, these pH-responsive samples have great potential in the applications of drug carrier.
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