本實驗之目的在於製備出含有金屬銀粒子之奈米纖維薄膜並探討其抗菌之應用。聚乙烯醇 (PVA)，為一種不具毒性且含有大量羥基(-OH)的水溶性高分子聚合物，且可經由生物降解並對於對環境無毒害。而聚己內酯則具生物相容性(biocompatibility)以及生物可分解性(biodegradable)的特性，此二種高分子已為廣泛地應用在生醫領域，為本實驗所採用。
電紡絲技術所製備出的奈米纖維具有高比表面積、高機械強度、方便、成本低等優點，因此我們選用此技術並加以探討。在高分子聚乙烯醇的部分，我們將硝酸銀加入至聚乙烯醇中，利用電紡絲的技術，製備出纖維薄膜。分別討論在紫外光及氬氣電漿處理下的抗菌情形。而在聚己內酯的部分，利用電紡絲分別製備出聚己內酯纖維薄膜及合成奈米銀溶液後，將聚己內酯浸泡至合成的奈米銀溶液，並討論其抗菌的情形。
本實驗所製備出的聚乙烯醇-銀與聚己內酯-銀纖維薄膜在經由革蘭氏陽性的黃金葡萄球菌及革蘭氏陰性的大腸桿菌測試後，均展現了良好的抗菌效果，我們相信未來能將其應用於傷口敷料，及其相關的領域中。

In this study, we have prepared two different electrospinning fibers: polyvinyl alcohol (PVA) and polycaprolactone (PCL). The advantages of applying electrospinning for preparing fibers including the provided high surface area to volume ratio, ease of fiber functionalization, low cost, and easy for mass production. The aims of this thesis were to prepare antimicrobial fibrous mats and the strategies were to incorporate silver particles into the prepared electrospun fibers.
For electrospun PVA or (PVA+AgNO3), the resultant fibrous mats revealed nanostructure with the average fiber diameter of 300 nm. The reduction of Ag was facilitated by applying highly energetic sources such as UV irradiation and argon (Ar) plasma. The results showed that the addition of AgNO3 assisted effectively the inhibition again bacteria, even without the applications of the high energetic sources. The antibacterial effects were evaluated by measuring the inhibition zone diameters (IZD) from two different types of bacteria: E. coli. and S. aureus.
The fibers prepared from PCL revealed microstructure and the incorporation of silvers was assisted by preparing silver particulate solutions separately and to immerse the PCL fibrous mat in the silver particulate solutions. The results showed that only when the PCL fiber interacted with Ag solution of pH 3.0, there was particulate Ag found on the PCL fibers. Furthermore, the prepared (PCL+Ag) fibers revealed good antimicrobial activities. The developments of the fibrous mat with high surface area and antimicrobial effect possess potentials for applications in wound dressing and related fields.

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