研究生: |
黃善宥 Shan-You Huang |
---|---|
論文名稱: |
多功能單鏈高分子奈米粒子:易於合成、特異的雙親性及高效的靶向藥物傳遞 Multifunctional Single-Chain Polymer Nanoparticles : Facile Synthesis, Unique Amphiphilicity, and Hightly Efficient Targeted Drug Delivery |
指導教授: |
鄭智嘉
Chih-Chia Cheng |
口試委員: |
鄭智嘉
Chih-Chia Cheng 蔡協致 Hsieh-Chih Tsai 邱文英 Wen-Yen Chiu 謝永堂 Yeong-Tarng Shieh 張雍 Yung Chang |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 應用科技研究所 Graduate Institute of Applied Science and Technology |
論文出版年: | 2019 |
畢業學年度: | 107 |
語文別: | 中文 |
論文頁數: | 183 |
中文關鍵詞: | 單鏈高分子奈米粒子 、雙親性接枝共聚物 、靶向藥物傳輸 |
外文關鍵詞: | Single Chain Polmer Nanoparticles, Amphiphilic Graft Copolymer, Targeted Drug Delivery |
相關次數: | 點閱:219 下載:0 |
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合成多功能性高分子材料的新概念,透過兩親性自組裝能有效生成單鏈高分子奈米粒子(SCNPs),因此在模擬蛋白質折疊結構方面取得重大的進展。在本論文中,我們開發一巨分子量兩親性接枝共聚合物,其在水溶液或有機溶劑中能進行自發性自組裝,形成直徑約為10 - 15 nm尺度均勻且穩定的SCNPs。SCNPs具有許多獨特的物理性質,包括特異的兩親特性、極低的黏度、良好的生物相容性及在血清存在下具有高度的結構穩定性,並可調節SCNPs的藥物負載量和粒徑。更重要的是,體外實驗證實裝載藥物的SCNPs能通過內吞作用被腫瘤細胞內化,進而逐步轉移到細胞核,表明這種獨特的SCNPs可以安全有效地應用於靶向藥物傳輸系統。
A new concept in synthesis of multifunctional polymeric materials has enabled significant progress in mimicking the folded structures of proteins by the efficient generation of single-chain polymer nanoparticles (SCNPs) via amphiphilic self-assembly. In this thesis, we developed an ultra-high-molecular-weight amphiphilic graft copolymer, which undergoes spontaneous self-assembly in aqueous solution or organic solvents to form uniform, stable SCNPs with a diameter approximately 10−15 nm. The resulting SCNPs possess a number of unique physical properties, including unique amphiphilic property, extremely low viscosity, good biocompatibility and high micellar stability in the presence of serum, as well as tunable drug-loading capacity and particle size. More importantly, in vitro experiments demonstrated that drug-loaded SCNPs were internalized by tumor cells through endocytosis, then subsequently progressively translocated to the nucleus, indicating that this unique SCNP system may serve as a safe and effective nanocarrier for targeted intracellular drug delivery.
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