研究生: |
林建宏 Chien-Hong Lin |
---|---|
論文名稱: |
利用層接式自我組裝方法製備聚電解質層固定化於PBAT膜 Layer-by-layer self-assembly of polyelectrolyte multilayer immobilized on PBAT films |
指導教授: |
楊銘乾
Ming-Chien Yang |
口試委員: |
李振綱
Cheng-Kang Lee 王大銘 none |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2006 |
畢業學年度: | 94 |
語文別: | 中文 |
論文頁數: | 92 |
中文關鍵詞: | PBAT膜 、幾丁聚醣 、硫酸葡聚醣 、聚電解質 、層接式自我組裝 、血液相容性 、生物相容性 |
外文關鍵詞: | PBAT, hemocompatibility, chitosan, dextran sulfate, polyelectrolyte complex, layer-by-layer self-assembly, cytocompatibility |
相關次數: | 點閱:328 下載:0 |
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本論文將以PBAT膜(Poly(butylene adipate-co-terephthalate),利用臭氧活化方式,進行聚胺基表面接枝,於膜表面產生-NH2基團,然後利用硫酸葡聚醣 (dextran sulfate,DS)與幾丁聚醣(chitosan)以層接式自我組裝方法形成多層聚集,分別探討其表面改質後血液相容性、生物相容性,並且分析血液凝固性,以作為血液導管生醫材料之應用。
層接式(Layer-by-layer, LBL)自我組裝改質方式係利用物種帶正、負電荷的性質,經由靜電力互相吸引而自組合,形成多層聚電解質的結構。由FE-SEM 及AFM觀察可知聚電解質多層結構厚度隨改質次數增加而增加且粗糙度並會些微下降。由XPS及染料確認可得知chitosan 及 dextran sulfate 量也隨固化層增加有線性增加趨勢,如此更使親水性提高。而且由於硫酸化葡聚糖量增加更降低PBAT膜蛋白質與血小板吸附進而增長血液凝血時間。整體而言,層接式自我組裝改質方式具有方便性、經濟性、可控制性更是無毒性之表面改質方法,改質後PBAT膜在血液接觸性生醫材用途深具潛力。
In this study, PBAT film was treated with ozone, followed by graft-polymerization of N-vinylformamide (NVF), and hydrolysis to introduce an amino-group bearing surface. Subsequently, dextran sulfate (DS) (as an anti-adhesive agent) and chitosan (CS) (as an antibacterial agent) were alternatively deposited onto the aminolyzed PBAT films in a layer-by-layer assembly manner, thereby constructing anti-adhesive and antibacterial polyelectrolyte complex (PEC) multilayer films. The progressive buildup of the multilayer film was verified by dye staining and XPS. These multilayer films were characterized by contact-angle and atomic force microscopy (AFM). The in-vitro evaluation of hemocompatibility was performed by measuring the adsorption of human serum albumin (HSA) and platelet adhesion as well as the blood coagulation time. The results from SEM and AFM show that the surface roughness changed little with the deposition of PEC layers. The hydrophilicity was improved with the increase of the number of PEC layers. Furthermore, the coagulation time prolonged with the number of PEC layers. Thus this demonstrated an easy process to prepare an anti-adhesive, cytocompatible, and antibacterial surface, and will be useful for surface modification of cardiovascular devices.
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