本研究測試以鳥糞石作為培養微藻（小球藻）之肥料。實驗用之鳥糞石以磷酸銨和氯化鎂混合來製備，並測得其水中溶解度為0.2 mg/l，這個平衡只需10分鐘即達成。本研究設計七種不同含鳥糞石之配方，並以BBM（Bold’s Basal Media）為對照組，比較藻類生長情形。經過OD（光密度）測試，其中六種顯示增長，再挑出生長最好的四種反覆實驗，選出其中最佳者。結果發現最佳配方是與對照組最相近的一種。用鳥糞石培養的小球藻使用兩種不同的碳源進行測試：二氧化碳（10%）及空氣。結果發現，以二氧化碳培養者生長程度僅比只使用空氣者略高，但空氣中生長的藻類呈現黃色（通常視為不健康的指標）。經測試不同含量的鳥糞石對藻類培養的影響，發現雙倍與三倍含量的鳥糞石讓藻類生長期更長且達到較高的OD。最後將鳥糞石和雙倍含量鳥糞石與標準BBM和含雙倍劑量磷酸、氨、鎂之BBM（等同於雙倍鳥糞石含量之BBM）比較，發現儘管培養於BBM的藻類有最低的OD，但也同時擁有最高的TSS（總懸浮固體）；鳥糞石和雙倍含量鳥糞石之TSS大約相同，而兩倍鳥糞石含量之BBM有最低的TSS。至於以鳥糞石培養藻類OD較高但TSS較低之原因仍未知。粒徑測定顯示，不同培養基之藻類粒徑並無差別。藻類可用脂質含量測試結果，最高者為BBM，其次為鳥糞石，再次為雙倍含量鳥糞石，最低者為雙倍鳥糞石含量之BBM。綜上結果得到可用脂質濃度如下：BBM為3.98 g/l，鳥糞石為2.85 g/l，雙倍含量鳥糞石為1.85 g/l，雙倍鳥糞石含量之BBM為1.01 g/l。本研究發現使用鳥糞石替代BBM中的磷、鎂和氨可降低成本。經計算，肥料價格可降低97.8%，藻類生質生產成本降低6.7%，藻類生物燃料生產成本降低1.8%。

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