本實驗以同軸電噴射法(coaxial electrospraying)製備幾丁聚醣(chitosan)-聚乳酸(polylactide)之核殼式奈米粒子(core shell nanoparticle)。藉由調整電壓、流速及工作距離的影響找出最佳化奈米粒子的參數，並且探討接枝葉酸(folic acid)以及乳鐵蛋白(lactoferrin)後粒徑之變化量。在腦膠質瘤(glioma)與腦膠質(glia)細胞培養系統下發現，低濃度下的核殼式奈米粒子在接枝標靶分子前後都可在腦膠質瘤及腦膠質細胞上展現良好的生物相容性。
細胞攝入實驗中顯示，接枝葉酸分子後的奈米粒子可以更有效的標靶腦膠質瘤細胞，且在24小時就有細胞攝入的效果。由體外(in vitro)薑黃素釋放的實驗中可以發現核殼式奈米粒子中的聚乳酸外層可以延緩突釋行為(burst release)。
藉由包覆薑黃素(curcumin)的投藥實驗可以發現，有載體包覆的薑黃素對腦膠質癌細胞有較好毒殺效果，在接枝葉酸後可以更進一步的提升毒殺效率，且對腦膠質細胞並無明顯毒性。最後藉由體外的血腦屏障(blood brain barrier)穿透實驗中證實了在接枝乳鐵蛋白後，能夠透過乳鐵蛋白受器更有效的穿透血腦屏障。

In this study, chitosan-polylactide (PLA) coreshell nanoparticles were fabricated by coaxial electrospraying. The applied voltage, flow rate and working distance were optimized in this research. After conjugated with folic acid and lactoferrin, the diameter of nanoparticles was not significantly changed. The biocompatibility of nanoparticles with different concentrations were investigated by culturing U87MG (glioblastoma) and SVGp12(glial) cells. We found that nanoparticles with low concentration would not result in significant cytotoxicity, no matter there was surface modification on nanoparticles or not.
The nanoparticle conjugated folic acid showed a better efficiency in cellular uptake, which was especially significant for glioblastoma cells. Compared with chitosan nanoparticles, core shell nanopaticle would decrease the burst release due to the existence of PLA shell.
The curcumin encapsulated in nanoparticles showed better cytotocixity for glioblastoma cells than free curcumin. If we immobilized folic acid onto nanoparticles, the cytotoxic to U87MG was further promoted. However, curcumin loaded in nanoparticles with and without surface modification showed limited cytotoxicity to glial cells. Finally, the results of in vitro BBB permeability indicated that conjugated lactoferrin on nanoparticles surface enhanced the permeability of nanoparticles to cross BBB.

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