研究生: |
楊玉雪 Yu-Shiue Yang |
---|---|
論文名稱: |
微脂粒與Triton非離子型界面活性劑之交互作用 Interactions between Liposomes and Nonionic Surfactants |
指導教授: |
陳崇賢
Chorng-Shyan Chern |
口試委員: |
李振綱
Cheng-Kang Lee 邱信程 Hsin-Cheng Chiu |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2005 |
畢業學年度: | 93 |
語文別: | 中文 |
論文頁數: | 77 |
中文關鍵詞: | 微脂粒 、非離子型界面活性劑 |
外文關鍵詞: | nonionic surfactant, liposomes |
相關次數: | 點閱:310 下載:2 |
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本實驗是利用超音波震盪法製備含有30 mole %的微脂粒並加入具有不同親水基鏈長的界面活性劑來探討界面活性劑與微脂粒之間的交互作用。藉由添加不同濃度之界面活性劑Triton X-100、Triton X-102、Triton X-165來探討界面活性劑由嵌入微脂粒脂雙層到瓦解微脂粒脂雙層的機制。首先,我們利用安定比來了解微脂粒在嵌入階段以及微脂粒脂雙層被瓦解的程序有何不同?且由UV-visible吸收值隨時間之變化情形得知不同的界面活性劑對微脂粒有不同的溶解能力。其次,我們以界面活性劑在微脂粒脂雙層中和水相中的分佈係數(K值)來探討Triton X-100、Triton X-102、Triton X-165嵌入微脂粒脂雙層中的情形,也了解不同界面活性劑對同一種微脂粒的嵌入情形也有所不同。最後,我們則使用1H-NMR來進一步了解界面活性劑嵌入微脂粒脂雙層以及微脂粒脂雙層漸漸被瓦解的機制。另外我們還有由Triton X-100、Triton X-102、Triton X-165的CMC值來探討三者之間的特性。
Liposomes comprising Egg PC and cholesterol (EPC : CH =7 : 3 ) were prepared by the sonication method. The objective of this work was to investigate the interactions between nonionic surfactants with different number of EO units of the hydrophilic part and liposomes. By adding different surfactants (Triton X-100, Triton X-102 or Triton X-165) to liposomes containing cholesterol, we studied the mechanism of inserting surfactants molecules into the bilayer of liposomes. First, we used the W values obtained from the dissolution kinetics experiments to study how the surfactant molecules were incorporated into the bilayer. Second, we used the K values (partition coefficient) to describe the portioning of surfactant molecules between the continuous aqueous phase and the bilayer. The conclusions obtained from the above experiments were further supported by the 1H-NMR spectra.
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