本研究以聚左旋乳酸(PLLA)為原料之微管陣列薄膜(microtube array membrane，MTAM)，進行基本性質分析與表面改質，增加細胞貼附與成長，以應用於生物反應器 (bioreactor)之開發與使用。本研究中首先以SEM拍攝MTAM外觀結構，計算平均其纖維直徑，並以PMI測量孔洞大小及分佈性，以拉力試驗測試MTAM強度，以熱重分析儀 (TGA)及示差掃描熱分析儀(DSC)測定熱裂解溫度及熱性質變化。接著以氧電漿進行表面改質，並使用EDC/NHS coupling reaction進行接枝精胺酸-甘胺酸-天冬胺酸(RGD)三肽，以接觸角比較接枝前後之改變情況，以傅立葉傳換紅外光光譜儀(FT-IR)判斷RGD胜肽之接枝之官能基，以UV分析計算RGD胜肽接枝密度。最後進行細胞相容性測試，將纖維母細胞 (L929)培養於MTAM表面，進行細胞增生測試。
生物反應器係以內徑16mm之聚甲基丙烯酸甲酯(PMMA)透明圓管作為外殼，用3D列印PLA製作黏接套件，以環氧樹脂組合成模組。其細胞毒性以L929纖維母細胞評估其生物相容性。生物反應器之效能，係於MTAM中空管狀處通入氣體，將纖維母細胞培養於MTAM表面，結果顯示細胞之增生率隨MTAM層數而增。因此，此MTAM生物反應器未來將可應用於生物性人工肝臟之領域。

In this study, PLLA was electrospun into microtube array membrane (MTAM). The resulting MTAM was examined using SEM and PMI to calculate average fiber diameter and pore size, using tensile test to determine mechanical strength, using TGA and DSC to measure pyrolysis temperature and thermal properties. The surface of MTAM was treated by oxygen plasma to improve fibroblasts (L929) attachment and growth, followd by using EDC/NHS coupling reaction to graft RGD peptide on MTAM surfuce, and employing Fourier transform infrared spectroscopy (FT-IR) to determine the amide group, and dyeing test to measure the graft density, and using contact angle to determine hydrophilic properties. The biocompatibility was evaluated with fibroblasts.
The housing of the bioreactor was made of PMMA transparent tube with inner diameter of 16mm. The PLA joints were fabricated by 3D printing and bonded with epoxy resins. The cytocompatibility was evaluated with fibroblasts. The performance of the bioreactor was evaluated by passing gas was put through the lumens of the microtube, and fibroblasts were seeded in the MTAM surface. The results show that the proliferation of fibroblasts increased with the number of MTAM layers. Thus this demonstrated the feasibility of MTAM bioreactor for bioartificial liver.

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