近來有奈米複合材料是非常廣泛且實用的研究題材，本實驗我們選用氧化石墨烯當主要應用材料，其表面富含氧化合物能與各種材料做結合，而氧化石墨烯在水中良好的分散性能避免氧化石墨烯聚集成大顆粒。而我們有興趣的是將氧化石墨烯當作載體去結合樹枝狀高分子聚合物，比較物理吸附與化學吸附的差異性。同時改變水溶液酸鹼值來分析其結合效果。結果顯示化學鍵結是比較有利於樹枝狀高分子吸附在氧化石墨烯上，同時改變酸鹼條件去影響吸附情形時，結果顯示在中性條件下結合程度最好，而酸或鹼的條件下則會降低樹枝狀高分子聚合物吸附的數量。此外我們更進一步想要了解單位氧化石墨烯最多能乘載多少樹枝狀高分子聚合物，其最大的吸附比重約是氧化石墨烯比樹枝狀高分子聚合物約為1 : 1.5，氧化石墨烯與樹枝狀高分子聚合物吸附體積比約是為1 : 2，氧化石墨烯的表面被樹枝狀高分子聚合物覆蓋率約為100%。藉由此結論往後我們將能藉此調配出最佳的比例。
修飾氧化石墨烯表面亦是一個相當重要的研究方向，本實驗將GO藉由超波音震盪方式製成奈米氧化石墨烯，其奈米氧化石墨烯片狀約為100奈米。我們選擇用-OH官能基的樹枝狀高分子聚合物與奈米氧化石墨烯作化學鍵結，結果顯示在樹狀高分子聚合物結合與氧化石墨烯結合後也能均勻地分散在水溶液中。氧化石墨烯由於其表面涵蓋豐富氧化合物，使得表面的界面電位呈現負值，我們藉由界面電位分析觀察加入樹狀高分子聚合物後其界面電位的改變。本文策略是奈米氧化石墨烯作為生物應用在藥物傳遞系統上。奈米氧化石墨烯加入草酸來當作癌細胞識別結合的官能基，以及搭載我們的抗癌藥物阿徽素，因為它的芳香族結構能高效率地搭載到奈米氧化石墨烯上面。最後結果顯示羥基之樹枝狀高分子有效地改善氧化石墨烯與草酸及阿徽素複合體的分散性。這顯示作為藥物傳遞系統奈米氧化石墨烯與羥基之樹枝狀高分子搭載草酸及阿徽素是可以被應用的。

In recent years, nano-composites become extensive and functional research objects. In this study, graphene oxide (GO) is focused as a main targeting material. Its surface has rich oxygen-based functional groups and can interact with variety of chemicals. Moreover, GO can be dispersed in water and avoid to self-aggregate into large particles. Due to these merits, GO can be a good candidate to hybridize with other materials in aqueous solution. The NH2-terminated Poly(amido amine) (PAMAM) dendrimer is preferably used to combine with surface functional groups of GO, to be a nano-composite, and then the hybrids of adsorption and chemical binding between GO and dendrimer are compared. Simultaneously, the immobilization effect depending on the solution pH is analyzed. The results show that the chemical binding procedure is preferable to immobilize dendrimer on GO in comparison with the adsorption procedure. Moreover, the immobilization is the best in the neutral condition and less at acidic or alkaline conditions. In addition, it is even likeable to know what the maximum quantity of PAMAM dendrimer can be immobilized on unit GO. The weight ratio of GO : dendrimer at saturated binding is about 1 to 1.5. In other definition, the optimum volume ratio of GO : PAMAM dendrimer is about 1 to 2, and then the total (about 100 %) surface of graphene oxide is covered by PAMAM dendrimer.
Surface modification of GO is also a very important research direction. In this study, nano-graphene oxide (NGO), i.e., a graphene oxide sheet of 100 nanometers in lateral width, has been prepared by ultra-sonication. Then NGO is more dispersed than GO in water. The OH-terminated PAMAM dendrimer was chemically immobilized with NGO and the results show that the hybrids of NGO and dendrimer can uniformly disperse in water. Herein, the strategy is biological applications of NGO as drug delivery system. NGO was conjugated with folic acid (FA) molecules for targeting specific cell with folate receptors. Furthermore, doxorubicin (DOX) was selected as an anticancer drug in this study, because its aromatic moiety can be loaded onto NGO with high efficiency. Finally, the results show that the OH-terminated dendrimer efficiently improves the dispersion of NGO-dendrimer/FA/DOX hybrid in water unlike the hybrids without dendrimer. This indicates the possible application of the NGO-dendrimer/FA/DOX hybrid to drug delivery systems.

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