我們成功製備了金奈米粒子
/奈米黏土之奈米複合物應用於表面
增強拉曼散射（ SERS）生物傳感器。奈米雲母通過親水聚合物
Jeffamine ED-2003 雙親性 聚合物 PIB-ED2003進行改 質 ，並用於穩
定金奈米粒子的成長。我們分別探討了插層、脫層以及改變親疏水性
之 SERS基板對於細菌的檢測的影響。脫層的 SERS基板透過自組裝
的方式將 AuNPs還原在基板上產生 3D熱點效應增強拉曼訊號。從
穿透式電子顯微鏡 (transmission Electron Microscope, TEM)可以明顯
看見奈米雲母片上的金粒子大小約為 5~10 nm之間，由於奈米雲母
片的厚度僅僅只有 1 nm的厚度，導致雙面吸附於奈米雲母片上的金
奈米粒子可以產生良好的 z軸 -方向 3D熱點效應。藉由檢測 DNA中
的生物分子 adenine可以展現出良好的拉曼增強因子 (enhancement factor, EF)可達 8.9 × 106 並且偵測極限可達 10-8M 可以有效且快
速的檢測出人型葡萄球菌及大腸桿菌 希望未來能夠 應用於 快速檢測
敗血症之相關細菌及食品安全中常見之菌種。

The nanoparticle arrays of Au-nanoclay were successfully fabricated for surface-enhanced Raman scattering (SERS) biosensing.The Mica clays were modified by hydrophilic polymer Jeffamine ED-2003,amphiphilic polymer PIB-ED2003 and utilize to immobile gold nanoparticles. We explored the effects of intercalation, exfolitaion , and the modification of hydrophilic and hydrophobic SERS substrates on the detection of bacteria.The hydrophilic nanohybird SERS substrate display the huge Raman enhancement by 3D hot spots produced from self-assembly of AuNPs on the nanoclays. The characterizations of Au-nanoclays would be evaluated by transmission electron microscopy, X-ray diffraction, Zeta potential and Raman spectroscopy using a 633 nm laser. The result shows that the diameter of Au nanoparticles is 5-10nm and the size nanoclays is about 500 nm × 500 nm x 1 nm. Furthermore, the hybrid substrate films formed three-dimensional (3D) hot-junctions and exhibited an SERS enhancement factor (EF) of 8.9 × 106 and limit of detection is 10-8M toward adenine molecules from DNA, which served as a model biomolecular target. The bacteria (S.H and E.Coli) could be quickly and sensitively detected by the flexible Au-nanoclays SERS substrate using Raman spectroscopy. It would be anticipated to further apply to rapidly detect Septicemia and food safety field.

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