近年來，電漿水在生物醫學領域逐漸受到重視，特別是在癌症治療方面。電漿水作為一間接治療方法，主要是透過生成活性氧及活性氮物質 (Reactive oxygen and nitrogen species, RONS) 來對癌細胞活性產生影響。然而，直至目前為止，RONS於細胞中所產生的特殊機制仍尚未被完全釐清。
本研究中將以去離子水置於大氣電漿中以置備出電漿水，同時藉由光譜分析儀確認電漿水中氮氣及氬氣的反應性離子之存在。此外，於電漿水置備完成後觀察到pH值之下降，此結果顯示電漿治療的效果。隨後，又進行了電漿水中長半衰期之RONS之濃度，例如硝酸鹽、亞硝酸鹽、過氧化氫以證明RONS可以保存於電漿水中至少七天的活性。
為了評估電漿水對於骨母細胞及骨癌細胞之影響，本研究利用電漿水進行了細胞活性測試，並採取不同電漿處理時間 (6 min、10 min、14 min、18 min、20 min) 及不同電漿水添加量 (80 μL、100 μL、200 μL、300 μL、400 μL) 與細胞培養液混合。根據結果顯示，相比骨母細胞電漿水更大程度地抑制了骨癌細胞的活性，且於最佳化條件的培養下，骨母細胞維持了細胞的增殖能力，然而骨癌細胞展現了明顯的細胞凋亡。在細胞膜電位差中，骨細胞皆發生了變化，其中骨癌細胞之膜電位差在電漿水的培養後明顯產生下降，意味著細胞膜完整性的喪失。此外於細胞型態的變化中觀察到，骨癌細胞的型態由紡錘狀轉變為球型近一步證明了骨癌細胞的凋亡。
儘管如此，電漿水並沒有影響骨母細胞之細胞活性反而提升了骨分化之表現，本研究通過細胞膜電位差之結果證明了電漿水對於骨癌細胞的細胞毒性，並對未來臨床癌症治療提供了較大的潛能。
 

Recently, plasma-treated water (PTW) has garnered considerable attention in biomedical fields, particularly in cancer treatment. PTW, as an indirect treatment method, primarily affects cancer cells through the generation of reactive oxygen and nitrogen species (RONS). However, the specific mechanisms by which RONS influence cellular fate have not been clearly identified thus far.
In our research, we prepared PTW by subjecting deionized water (DI water) to atmospheric pressure plasma. Through optical emission spectroscopy, we confirmed the presence of nitrogen and argon reactive ions in the PTW. Furthermore, we observed a decrease in the pH value, which indicated the effectiveness of the plasma treatment. Subsequently, we measured the concentration of long-lived RONS, such as nitrite, nitrate, and hydrogen peroxide, providing evidence that these RONS can persist for at least 7 days.
To assess the impact of PTW on osteogenic cells and osteosarcomas, we conducted viability tests using PTW with varying plasma treatment times (6 min, 10 min, 14 min, 18 min, 20 min) and different volumes (80 μL, 100 μL, 200 μL, 300 μL, 400 μL) added to the culture medium. The results demonstrated that PTW suppressed osteosarcoma viability to a greater extent than that of osteogenic cells. Under optimized conditions, osteogenic cells maintained their proliferation, while osteosarcomas underwent clear apoptosis. Alterations in the membrane potential of both osteoblasts and osteosarcomas indicated a significant decrease in the membrane potential of osteosarcomas upon exposure to PTW, signifying the loss of membrane integrity. Additionally, the morphological changes observed, where spindle-shaped cells transformed into spherical cells, further supported evidence of apoptosis in osteosarcoma.
Conversely, PTW did not impact the viability of osteoblasts and even promoted their osteogenic differentiation. This study provided evidence that PTW exhibited greater cytotoxicity towards osteosarcoma cells, which correlated with the permeability of the cell membrane. These findings offer potential clinical applications of PTW in cancer treatment.

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