研究生: |
吳仲翔 Chung-Hsan Wu |
---|---|
論文名稱: |
利用Yarrowia lipolytica 生產生質酒精 Bioethanol production from Yarrowia lipolytica Po1g biomass |
指導教授: |
朱義旭
Yi-Hsu Ju |
口試委員: |
王孟菊
Meng-Jiy Wang 李文乾 none |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2012 |
畢業學年度: | 100 |
語文別: | 英文 |
論文頁數: | 46 |
中文關鍵詞: | Sacchromyces cerevisiae 、Yarrowia lipolytica 、水解 、酒精 |
外文關鍵詞: | Sacchromyces cerevisiae, Yarrowia lipolytica, Hydrolysis, ethanol |
相關次數: | 點閱:189 下載:14 |
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生質酒精發展到現在已經是一個重要的替代能源。在這研究中,我們使用Yarrowia lipolytica酵母菌的菌體來產生質酒精。探討乾菌與酸溶液的比例(1:8-1:15)、水解溫度(90-150℃)以及硫酸濃度(2-15% w/w)在固定水解反應時間為1小時下對於水解結果的影響。取乾菌與酸溶液為1:10、以6wt%的硫酸溶液在120℃並且使用去脂乾菌來進行水解,可以得到最大的葡萄糖量,濃度與產率分別為35.89 g/L。使用次臨界水前處理對於葡萄糖產量有負面的影響﹔若使用未經去脂處理的乾菌來水解,以6wt%的硫酸溶液在120℃水解條件下只能得到14.53 g/L的葡萄糖。在此研究中,可得到的最大酒精濃度為13.39 g/L;產率為0.084克酒精/克乾菌重(0.38克酒精/克葡萄糖)。
Bioethanol has become an important alternative energy source. Bioethanol production from the yeast Yarrowia lipolytica biomass was studied. The effects of biomass to acid solution ratio (1:8 – 1:15) temperature (90 - 150oC) and H2SO4 concentration (2 - 15% w/w) on the saccharification of biomass at a hydrolysis time of 1 h were investigated. A maximum glucose concentration of 35.89 g/L can be produced from defatted biomass, biomass to acid solution ratio equal to 1:10 and using 6% H2SO4 at 120oC. Subcritical water (SCW) pretreatment has negligible effect on maximum glucose concentration achievable. Only 14.53 g/L glucose can be produced using 6% H2SO4 at 120oC if un-defatted biomass was used. The highest ethanol concentration achieved was 13.39 g/L with a corresponding ethanol yield of 0.084 g/g dry biomass (0.38 g ethanol/g glucose).
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