本論文改良傳統的檸檬酸鈉熱還原法 (Frens方法)合成出分散性更佳的金奈米粒子 (gold nanoparticles，Au NPs)，其關鍵在於還原四氯金酸 (HAuCl4)前的反應液中加入了一低成本、天然、環保的植酸鹽 (inositol hexaphosphate，IP6)。結果顯示，金奈米粒子的粒徑與型態會隨著IP6濃度 (0~32 M)有所變化，將藉由吸收光譜與反應動力學來觀察探討此現象。此外，植酸鹽濃度等於6.5 M時，因其優異的吸附能力，植酸鹽會於金奈米粒子表面形成明顯的植酸鹽層，而組成核 (Au NPs)-殼 (IP6)結構。因此植酸鹽層不但可以使金奈米粒子有更良好的分散效果，也可作為奈米金粒子間的分隔層，使彼此能固定於數奈米的間距 (1~2 nm)，形成非常強烈的熱點效應 (hot spots effect)，在表面增強拉曼光譜 (surface-enhanced Raman scattering，SERS)檢測上，具有相當大的應用價值，例如微生物 (金黃色葡萄球菌)的生物檢測。

We have developed a method to synthesize gold nanoparticles (AuNPs) by adding an environment-friendly inositol hexaphosphate (IP6) to the reaction solutions before reducing HAuCl4 through the traditional citrate thermal reduction method (Frens method). The results show that the morphology and the particle size of Au NPs would dramatically be varied with the concentration of IP6 (0~32 M). Furthermore, the core (AuNPs)-shell (IP6 layer) structure was found in 6.5 M IP6 due to the excellent adsorption capability of IP6. It not only makes AuNPs disperse well but also separates particles in a specific distance (1~2 nm) due to the ionic repellence of IP6. The strong hot spots effect was observed in the core-shell structure, which would have a great applicability in the surface-enhanced Raman scattering (SERS) detection, such as microoganism biosensing of Staphylococcus aureus (S. aureus).

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