以基因重組大腸桿菌生產的水溶性多精胺酸聚天門冬胺酸，為一親水性及具有生物相容性的多胜肽，本研究利用此多胜肽作為穩定劑，在還原劑的作用下，以簡易快速的方法合成具高穩定性及生物相容性的貴金屬奈米粒子。
經由穿透式電子顯微鏡觀察到5 nm以下且形狀多為球型的奈米粒子，並利用X射線光電子能譜儀 (XPS)、傅立葉轉換紅外線光譜儀 (FTIR)、能量散射X射線能譜儀 (EDX) 及凝膠電泳對水溶性多精胺酸聚天門冬胺酸穩定的貴金屬奈米粒子 (multi-L-arginyl-poly-L-aspartate-stabilized noble metal nanoparticles, sMAPA-noble metalNPs) 進行特性分析，探討其鍵結方式、成分及電荷。
應用上探討了sMAPA-noble metalNPs在近紅外光照射下的光熱效應，以及對偶氮染料甲基橙及亞甲藍降解的催化，並計算反應速率常數及活化能，其中以鈀奈米的催化能力最為優秀。同時以MTT assay測量不同濃度的sMAPA-noble metalNPs對纖維母細胞L929的細胞毒性。
此研究合成小於5 nm的貴金屬奈米粒子，並具備良好的生物相容性及催化能力，使其在生醫材料及偶氮染料降解催化獲得巨大潛力。

Soluble multi-L-arginyl-poly-L-aspartate produced by recombinant Escherichia coli is a hydrophilic and biocompatible polypeptide. In this study, the polypeptide was used as a stabilizer in a simple and rapid method for the synthesis of noble metal nanoparticles with high stability and biocompatibility in the presence of a reducing agent.
The synthesized nanoparticles below 5 nm and mostly spherical in shape were observed by transmission electron microscopy (TEM). Soluble multi-L-arginyl-poly-L-aspartate-stabilized noble metal nanoparticles (sMAPA-noble metalNPs) were characterized by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), and gel electrophoresis.
For the applications, the photothermal effect of sMAPA-noble metalNPs under near-infrared light irradiation was discussed as well as the utilization as a catalyst for the degradation of two azo dyes, methyl orange and methylene blue. The reaction rate constant and activation energy were estimated. Among those sMAPA-noble metalNPs, palladium nanoparticles showed a high catalytic efficiency. Finally, the cytotoxicity of different concentrations of sMAPA-noble metalNPs on fibroblast L929 was tested by MTT assay.
In this study, noble metal nanoparticles blow 5 nm were synthesized and showed good biocompatibility and catalytic efficiency, which make them great potential as a composite biomaterial and catalyst for the degradation of azo dyes.

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