本實驗分為兩個部份，第一部分以共沈澱法合成出粒徑約為20~30 nm之四氧化三鐵粒子 (Iron oxide nanoparticles, Fe3O4，FeNPs) 。接著將FeNP改質後，加入Protein G連接，接著透過Protein G與IgG抗體的生物親和性結合後，使抗體能與抗原反應，續與帶有螢光標籤的二抗反應後，以雷射共軛焦顯微鏡觀察確認反應之成功性。
第二部分為了進一步對HDL的分離做統計分析，透過定量之BSA檢量線來推論出磁珠之穩定抓取濃度，並與傳統的高速離心法比較，找出最適合應用於臨床HDL分離之奈米磁珠實驗參數。
對此，本研究尋求一個能取代舊有的純化HDL的方式，藉由磁珠分離具有快速且能夠檢驗大量樣本檢體之特性，冀期能在未來做出可快速篩檢的檢測HDL功能的檢測工具。

The thesis consists of two parts, including synthesizing Fe3O4 @APTES@ProteinG-Ig nanoparticles, measurement of HDL oxidative fluorescence units by detecting emmison wavelength of DHR. First, iron oxide nanoparticles (FeNPs) with a mean diameter 25nm is prepared by co-precipitation method, then modifying on FeNPs’ surface to synthesize FeNPs. Next, by coating Protein G on FeNP@APTES nanoparticles’ surface, we have succefully synthesized FeNP@APTES@Protein G-Ig structure by protein G bio-affinity.
Second, the composite particles were characterized with different HDL concentration, including specificity capturing HDL observed by CLSM, capturing amount deduced by applying UV method, and linear regression with tratitional ultracentrifugation and DCFH flurescent detection method etc.
This study aims to provide a new novel method to substitute for ultracentrifugation method which takes 6 days to separate pure HDL from serum. Magnetic beads have known for its rapid separation ability with good bio-compatibility. We find highly potential of magnetic beads in large clinical trail of HDL quality examination.

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