本研究中，我們製備多孔型水膠/光固化樹酯複合支架，目的是以和藥
物相容性高且釋放率高的水膠作為藥物載體進行藥物釋放，藉由光固化樹 酯支架有效的控制孔洞結構進而影響物質的釋放行為。
許多研究結果顯示結構的孔徑與孔隙率會影響藥物釋放，然而通過傳 統多孔結構製備方法難以精確控制結構的孔徑和孔隙率。在本研究中，我們 使用光固化 3D 列印技術開發具有高精細度的孔洞結構支架，並將水膠作為 藥物載體填入光固化樹酯支架中進行藥物釋放，以系統化地分析結構因子 對藥物釋放的影響。在這項研究中，設計了兩種支架結構(直通道和相互連 接的孔洞)，同時分別使用了三種孔徑(分別為 4mm、3.46mm 和 2.83 mm)、 三種孔隙率(分別為 52.6%，62.9%和 80.2%)以及三種扭曲率(分別為 1m/m，1.2m/m 和 1.5m/m)作為設計參數，將含有亞甲基藍的水凝膠填充到 支架中，並在各個時間點測量釋放曲線，從釋放曲線來看，與無支架的水凝 膠直接釋放相比，釋放速率和爆發釋放降低至約 40%和 60%，此結果顯示 多孔結構有效地延長了釋放時間。
接著統計及分析各孔洞參數對釋放速率的影響，結果顯示在前期的釋放 中，釋放速率隨結構孔徑與孔隙度而增加，中期的釋放速率略隨孔隙度的上 升而下降，而後期釋放時程則明顯地因扭曲度的上升而拉長。我們進一步建
I
立出一套數學模型，可利用此模型設計所需要的釋放行為，並且在結構上增 加了導管設計，可以優化在臨床應用上更換藥物或填補含藥水膠的便利性。
作為骨移植材料，結構需要一定的機械強度，而本研究設計的多孔結構 支架在使用 PC 寡聚物時其楊氏係數為 0.4 GPa 至 0.8 GPa，機械性能非常 接近鬆質骨，且此材料具有良好的生物相容性，這表明本研究開發的具有藥 物遞送系統的支架在骨組織工程中具有很高的潛力。

In this research, porous hydrogel/photo-resin composite scaffolds were prepared. The hydrogel with high drug compatibility and excellent releasing behaviors were applied to encapsulate and release drugs, and 3D printed photoresin would provide precise porous structures for controlled delivery.
It was indicated that the pore sizes and porosity of carriers would affect the drug delivery in previous studies. However, it was difficult to control porous structures precisely by using convectional processes. The photo-curing 3D printing technique was applied in this study to create well-designed porous structures, and the hydrogel pore-fillers were used for drug release. Thus, the effect of structure parameters can be systematically investigated. We designed two structures (straight and interconnected structures), three pore sizes (4, 3.46 and 2.83 mm), three porosities (52.6, 62.9, and 80.2%) and three tortuosity (1, 1.2 and 1.5 m/m) for 3D printed scaffolds. The hydrogel containing methyl blue was then filled into resin scaffolds and the released amount was measured at various time points. The profile presented that the delivery rate and burst release were decreased by about 40% and 60%. It proved that the porous structures effectively prolong the releasing period.
The structure effects on release rates were statistically analyzed. The results revealed that the early release rate increased with pore sizes and porosity, the release rate in the middle stage decayed with the increase in porosity, and the late release was prolonged with the increase in tortuosity. Based on the statistical analysis, a mathematical model was developed, which was applicable in customizing drug delivery. Besides, the in vitro injection channel developed in this study would be able to promote the convenience in adding or replacing the hydrogel clinically.
As a bone graft, the Young’s modulus of porous scaffolds developed in this research was about 0.4 -0.8 GPa, which was close to the sponge bones. Moreovet, the biocompatibility of photo-resin and hydrogel were high. The results identified the potential of hydrogel/photoresin composite scaffolds in bone tissue engineering.

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