目前許多研究指出，聽力損傷及內耳平衡失調皆與內耳毛細胞受損有關，最近，內耳基因治療，利用插入帶有功能型蛋白之基因序列，使得內耳毛細胞得以保存，並且受到保護，或使毛細胞再生。於先前的研究中，本研究團隊以未來應用於臨床為基礎，證實超音波結合自製微氣泡對比劑之技術於圓窗膜進行內耳藥物釋放的可行性，而本研究將以超音波結合微氣泡對比劑之技術，搭配微脂體之基因載體為基礎，探討不同粒徑大小的微氣泡於內耳細胞進行基因治療之可行性。根據實驗結果，分別增加對比劑成分中葡萄糖及白蛋白濃度，皆可提升微氣泡之粒徑。於相同的超音波聲場強度下，0.66 μm、1.32 μm及2.83μm之白蛋白微氣泡對比劑，基因轉殖效率提升了，30.6%，54.1%及84.7%；1.39 μm、2.12 μm及3.47 μm之葡萄糖白蛋白微氣泡對比劑，基因轉殖效率提升了，15.9%，34.3%及82.7%。本研究成功利用增加微氣泡粒徑來控制微氣泡彈性的特性，而生產出超音波結合微氣泡對比劑進行基因轉殖之理想微氣泡，並確定使用超音波進行有效的基因轉殖之中，微氣泡粒徑對於其振盪行為的重要性，以及評估出使用超音波進行內耳細胞基因轉殖之最佳微氣泡粒徑。

Hearing loss is the most common sensory impairment in humans, and the causes are dysfunction, injury, or death of the hair cell. Recently, gene therapy has been reported to insert genes encoding functional proteins that can preserve, protect, or regenerate hair cells in the inner ear. In our previous study, a basis for microbubbles (MBs) ultrasound (US)-mediated techniques with therapeutic medication delivery to the inner ear for future application in humans has been demonstrated. In this study, cationic liposomes were as the delivery vectors and the feasibility of albumin MBs US-mediated techniques in different sizes MBs with gene delivery to the inner cells has been investigated. According to the results, the MBs size was in direct proportion to the increase of the concentration of albumin or dextrose, respectively. With the same US energy, the transfection of albumin MBs with 0.66, 1.32, 2.83 μm in diameter increase 30.6% , 54.1% and 84.7% . The transfection of dextrose albumin MBs with 1.39, 2.12, 3.47μm in diameter increase 15.9%, 34.3% and 82.7%. To increase MB size and control property of elasticity produce desired levels of US mediated gene transfection. The MBs sizes in different condition have been determined and their oscillation behavior is important for effective gene transfection using US. The optimal MBs size for gene transfection in the inner ear cells with US has been evaluated.

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