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研究生： Okta Pianti Rahayu
Okta Pianti Rahayu
論文名稱： 溶劑對偶聯劑合成的影響及其在電化學免疫傳感器CA125檢測中的應用
An Investigation of Solvent Effect on The Synthesis Of Coupling Agent and Its Application To The CA125 Detection of Electrochemical Immunosensor
指導教授： 郭俞麟
Yu-Lin Kuo
王復民
Fu-Ming Wang
口試委員: 郭俞麟
王復民
白孟宜
學位類別： 碩士
Master
系所名稱： 工程學院 - 機械工程系
Department of Mechanical Engineering
論文出版年： 2022
畢業學年度： 110
語文別： 英文
論文頁數： 66
中文關鍵詞： rGO 、CA125 、乙醇 、甲苯 、APTES
外文關鍵詞： Electrochemical Immunosensors
相關次數： 點閱：135  下載：0 
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    • Ovarian cancer cases keep increasing and become one of the most lethal cases worldwide. The detection of ovarian cancer usually after the main symptoms appeared and the person will be diagnosed with a high level of ovarian cancer afterward. In this case, the early detection of malignancy is needed in the treatment of ovarian cancer. Herein, we report on the development of an electrochemical immunosensor for the early detection of carcinoma antigen 125 (CA125). The graphene oxide was firstly synthesized with APTES, the APTES was the silanization agent which could functionalize the rGO. APTES was grafted to rGO through synthesis process, the process was using two different solvents; Ethanol and Toluene. APTES could cross-link in solution and that cross-linked layer could grafted the surface of rGO. The best solvent was one where hydrolysis and self-condensation do not occur. Consecutively, it was conjugated with anti - CA125 monoclonal antibody (Ab) using an intermolecular crosslinking reaction. The nonspecfic binding sites were followed by enclosed wtih bovine serum albumin (BSA). Differential pulse voltammetry was used to investigate the electrochemical properties of the developed BSA/Ab/rGO@APTES-SA/Thi immunosensor. The electrochemical immunosensor offers a good response for the detection of CA125 antigen with a linear range (5x10-5 - 0.033 µg/mL). The sensor that used rGO@APTES-SA by Ethanol not really sensitive to detect CA125 due to the condensation happened in ethanol which affecting the performance of surface functionalization. Whereas in toluene, APTES condensation reaction was not significant, allowed a greater availability of APTES molecules to react with the rGO, resulted in a more efficient functionalization. Based on the simple synthesis process of rGO and high sensitivity of the developed immunosensor, the developed immunosensor will provide a sensitive, convenient approach to be used for detection of CA125 antigen or even the early detection and treatment of ovarian cancer (OC).

    Abstract ii TABLE OF CONTENTS iii LIST OF PICTURES v CHAPTER 1 1 INTRODUCTION 1 CHAPTER II 4 LITERATURE REVIEW 4 2.1 Ovarian cancer 4 2.1.1 Geographical statistics 6 2.1.2 Pathology classification of OC 7 2.1.3 Tumor Marker of Ovarian Cancer 10 2.2 Immunoassay 11 2.2.1 Immunoassay Sandwich ELISA 12 2.3 Biosensor 13 2.3.1 Classifications of Biosensor 14 2.3.2 Electrochemical Biosensor 15 2.4 Carbon Black 16 2.5 Graphene 17 2.6 Thionine 19 2.7 APTES 20 2.8 Succinic Anhydride 24 2.9 Research Motivation 25 CHAPTER III 26 EXPERIMENTAL PROCEDURE 26 3.1. Materials and reagents 26 3.2. Instruments 27 3.3. Experimental procedures 27 3.3.1 Preparation of rGO@APTES-SA 27 3.3.2 Preparation of 1st and 2nd layer solution 28 3.3.3 Preparation flow of synthesized rGO 29 3.3.4 Differential Pulse Voltammetry (DPV) 32 3.3.5 Electrochemical measurement 34 3.3.6 TGA 35 3.3.7 SEM 36 CHAPTER IV 38 RESULTS AND DISCUSSION 38 4.1 The effect of carbon support 38 4.1.1 Effect of graphene modification on electrochemical responses 38 4.1.2 Effect of graphene functionalized by APTES-SA on electrochemical responses 39 4.2 Comparison of carbon materials by SEM 42 4.3 Evaluation after testing with Different concentration of CA125 43 4.4 Trial with serum with/without Ovarian Cancer (OC) 45 CHAPTER V 51 CONCLUSION AND FUTURE WORKS 51 REFERENCES 53

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