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研究生： 蕭至維
Chih-Wei Hsiao
論文名稱： 噴霧乾燥法製備生物活性玻璃粉末及其結構觀察之研究
Synthesis and characterization of spray dried bioactive glass
指導教授： 施劭儒
Shao-Ju Shih
口試委員: 施劭儒
Shao-Ju Shih
王丞浩
Chen-Hao Wang
顏怡文
Yee-Wen Yen
林穎志
Ying-Chih Lin
學位類別： 碩士
Master
系所名稱： 工程學院 - 材料科學與工程系
Department of Materials Science and Engineering
論文出版年： 2018
畢業學年度： 106
語文別： 中文
論文頁數： 95
中文關鍵詞： 生物活性玻璃 、氫氧基磷灰石 、噴霧乾燥法 、量產 、生物活性
外文關鍵詞： bioactive glass, hydroxyapatite, spray drying, mass production, bioactivity
相關次數： 點閱：404  下載：21 
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  •  上一筆

    • 隨著社會的老年化以及人們對於健康要求提高，生物活性玻璃(bioactive glass, BG)材料受到關注，BG在人體的體液中可以形成氫氧基磷灰石(hydroxyapatite, HA)，骨母細胞能夠將HA進行改質形成人體的骨頭，幫助骨頭修復。傳統玻璃製程和溶膠-凝膠法被廣泛應用在合成生物活性玻璃多年，然而這兩種方法各有其優缺點。首先，傳統玻璃製程需要相對高的製備溫度(1250-1400oC)，而且對於生物活性玻璃的純度有疑慮，因為需要進行研磨和過篩的階段過程中，可能會影響純度。再來是溶膠-凝膠法可以提供較低溫的製備溫度(600-700oC)，而且可以藉由合成手法來控制粉體成分以及微結構，進而影響粉體的生物活性，然而此方法卻需要花費長時間(數天)合成BG粉體，而且也較難將BG粉體進行量產。因此，本研究利用噴霧乾燥法(spray drying method, SD)來克服上述的缺點，SD法能夠短時間合成出高純度的BG粉體和具有量產化的特性(i.e. 250g/15min)。SD法合成BG粉體的研究報導少，所以將進行合成常見的58S、68S和76S BGs粉體，並且進行討論以SD法合成的粉體形貌的觀察以及生物活性的評估。

    利用X光繞射技術(XRD)分析BG粉體的相結構，並利用掃描電子顯微技術(SEM)觀察BG粉體的表面結構，並做體外的生物活性實驗，將BG粉體浸泡於模擬人體體液(simulated body fluid)12小時，利用XRD分析HA的相結構以及SEM觀察HA在BG表面的生成。經由實驗結果進行討論，調控前驅物溶液的pH值以及濃度，可以控制粉體的形貌，具有圓球形的(sphere)及皺褶的(crumpling)兩種形貌。比較BG成分，58S BG具有較佳的生物活性(HA生成量)，依序為68S 和76S BGs。

    Bioactive glasses (BGs) have received lots of attention from biologists and engineers because of their potential applications in bone implants. BGs will form hydroxyapatite (HA) structure in body fluid to help bone recovered. The conventional glass process and sol–gel process are popular methods for fabricating BGs, and have been used to produce BGs for years. However, they have some disadvantages of producing BGs. Firstly, the conventional glass process requires comparatively high formation temperature (1,250–1,400oC) and has difficulty fabricating high purity glasses for bioactive control due to grinding and sieving procedures. Secondly, although the sol-gel process offers a lower heat treatment (600–700oC) and provides a broader range and greater control of bioactivity because it can alter the BG composition or microstructure by manipulating processing parameters, it has a problem to have mass production. Therefore, this study presented a spray drying (SD) method to overcome the disadvantages of purity and mass production (i.e. 250g/15min). The common 58S, 68S and 76S bioactive glasses are synthesized by SD method, and measured their powder morphologies and observed thier bioactivities.

    The XRD technique analyzed the phase structures, and scanning electron microscopy measured the surface morphologies of the BG powders. In addition, in vitro bioactive tests showed the formation of HA layers on BG particles after immersion in simulated body fluid for 12h. Experimental results show the promising potential of SD method to fabricate BG particles and to control the spherical and crumpling morphologies of BG particles. From the results, 58S BG has the best bioactivity, and the second and the last are 68S and 76S BGs, respectively.

    摘要 I Abstract II 致謝 III 圖目錄 VIII 表目錄 XII 第一章 緒論 1 1.1 研究背景 1 1.2 研究動機 3 第二章 文獻回顧 4 2.1 生醫材料 4 2.1.1生醫金屬 5 2.1.2生醫高分子 5 2.1.3 生醫陶瓷 5 2.2 生物活性玻璃 8 2.2.1 生物活性玻璃的材料特性 9 2.2.2 生物活性的機制以及BG組成的影響 10 2.2.3 生物活性的分析 12 2.3 生物活性玻璃合成方式 16 2.3.1 傳統玻璃法 16 2.3.2 溶膠-凝膠法 18 2.3.3 噴霧熱裂解法 22 2.3.4 噴霧乾燥法 26 2.4 噴霧乾燥法 28 2.4.1 噴霧乾燥法之簡介 28 2.4.2 前驅物溶液之配製 29 2.4.3 噴霧乾燥粉體的形貌之影響分子 36 2.4.4 旋風集塵技術 41 2.5 研究目的與重要性 43 第三章 實驗目的與方法 44 3.1 實驗設計 44 3.2 實驗原料 46 3.3實驗儀器設備 47 3.4 樣品製備之步驟 48 3.5 樣品性質及分析方法 50 3.5.1 X光繞射儀 50 3.5.2 場發射掃描式電子顯微鏡 51 3.6 生物相容性 52 3.7 體外生物活性評估 53 第四章 結果 54 4.1 58S BG粉體之合成及分析 54 4.1.1 前驅物粉體熱處理及酸洗處理 54 4.1.2 前驅物溶液的pH值對粉體之影響 57 4.1.3 前驅物濃度對於顆粒尺寸之影響 63 4.1.4 生物毒性測試 67 4.2 58S、68S和78S BG粉體之合成及分析 69 4.2.1 58S、68S和76S BG之相分析 69 4.2.2 微結構形貌以及粒徑分布 70 4.2.3 BG粉體於SBF的pH值變化 73 4.2.4 生物活性測試 75 第五章 討論 77 5.1 顆粒形貌 77 5.1.1 前驅物溶液的pH值對於BG粉體形貌之影響 78 5.1.2 前驅物溶液的濃度對於BG粉體形貌之影響 80 5.2 生物活性 82 5.2.1 生物活性玻璃與pH值 82 5.2.2 生物活性玻璃與生物活性 83 5.2.3 生物活性與pH值 84 第六章 結論 86 第七章 未來工作 87 參考文獻 88

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