本研究探討組織玻片之自動化對焦與圖片縫合技術。自動化對焦有利於全自動玻片掃描機台的開發，而圖形比對縫合有利於改善圖片縫合後所產生的接縫 。系統使用CCD攝影機搭配倒立式顯微鏡擷取切片組織影像，並應用Sobel測邊遮罩來撰寫對焦程式。依據程式演算的結果，系統可自動調整機構平台上下位置使波片置於最佳焦距。完成對焦的玻片隨著機構平台橫向移動，並在指定的位置進行影像的掃瞄。玻片之橫移是利用音圈馬達驅動平台，位置精度可達0.2μm。生物玻片掃描結果經由影像處理程式的縫合，可組成一張高解析、高畫素的切片組織影像，提供醫療人員進行病理診斷或教育訓練之用。

This research studies the automatic focusing and image recomposing techniques for biological tissues. The former ones are essential to develop an automatic tissue scanning platform and the later ones can help to improve the quality of the stitched images. A CCD camera with microscope is used in the proposed system to collect partial images from a tissue specimen. The height of the specimen can be adjusted automatically by the system based on the results from an edge-detect program. After the tissue has been properly focused, it is moved horizontally with the platform and an image is taken after each constant displacement. The horizontal movement is drived by a SMAC linear moving coil actuator with repeatability equal to 0.2μm. With the assistance from the library provided by Labview, the image processing subroutines can effectly recompose a panoramic picture from the images on the same row. The resultant picture of the tissue specimen shows a good resolution and can be used to help medical diagnosis and education .

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