本實驗利用具有溫度敏感性聚(氮-異丙基丙烯醯胺)(Ploy(N-isopropylacrylamide)，PNIPAAm)以及具有生物相容性的明膠(gelatin)藉由不同粒徑大小的聚苯乙烯微球(polystyrene , PS)製備出各尺寸的有序多孔性結構薄膜。首先利用N,N'-Methylene bisacrylamide (MBA)將gelatin與PNIPAAm進行交聯後，再使用無乳化劑乳化聚合法聚合出四種粒徑的PS球，接著將四種粒徑大小的PS球加入其溶液中，再將此溶液乾燥成膜並浸泡於甲苯中去除PS球，最後可得四種不同尺寸的gelatin-PNIPAAM多孔性結構薄膜。
利用SEM以及DLS證實本實驗成功製備出不同粒徑大小的PS球，並由FTIR證實gelatin與PNIPAAm交聯，再經由SEM確定gelatin-PNIPAAm多孔性結構薄膜的製備完成，接著使用SEM和CA探討溫度變化對有序多孔性結構薄膜的影響，並在藥物釋放及抗菌測試實驗，證明多孔性結構薄膜在高於LCST的水溶液環境所造成的大孔洞能夠擁有較好的藥物裝載及釋放量，並對於細菌的生長有更好的抑制效果。

In this study, we developed to prepare thermosensitive Ploy(N-isopropylacrylamide)(PNIPAAm) with an biocompatible gelatin and various particle sizes of polystyrene microspheres for the purpose of synthesizing ordered porous film. N,N'-Methylene bisacrylamide (MBA) was used to crosslink gelatin and PNIPAAm. We also used emulsifier-free emulsion polymerization to prepare various particle sizes of monodisperse polystyrene microspheres. The polystyrene microspheres acted as a template to create various size ordered porous film.
Scanning electron microscope(SEM) and dynamic light scattering (DLS) demonstrated various diameters monodisperse polystyrene microspheres were synthesized successfully. The cross-linking reaction of gelatin-PNIAPAAm was confirmed by fourier transform infrared spectroscope(FTIR). And then SEM also revealed the structure of gelatin-PNIPAAm ordered porous film. The thermo-responsive properties of gelatin-PNIPAAm ordered porous film was investigated by SEM and contact angle(CA). Due to the thermo-responsive property of PNIPAAm, the drug release and antibacterial test can confirmed the large pore sizes of the film could have better amounts of drug release and load.

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