紫膜(purple membrane，PM)存在於Halobacterium salinarum古生嗜鹽桿菌中，膜上主要的蛋白質細菌性視紫質(bacteriorhodopsin，BR)具有光驅動質子泵的特性。本研究選用由長碳鏈磷羧酸所組成之自排性單層分子膜修飾ITO導電玻璃，再經化學改質後將PM以生物親和性吸附固定化。首先以水滴接觸角與循環伏安法分析觀察塗覆層間的差異，接觸角度會從ITO面的3~5。瞬間上升到單層分子膜的75。，並於後續的塗覆修飾中一路下滑至PM層的20~30。之間；循環伏安法分析則清楚顯示電極的氧化還原峰電流隨著塗覆層增加而明顯縮小。XPS元素分析也證明各塗覆層的存在。所製備PM晶片的光電流響應受PM懸浮塗覆液成分、晶片沖洗條件與電解液的酸鹼值強烈影響，最佳光電流密度最大可達1.1±0.1 μA/cm2。AFM與XPS分析顯示PM膜片於基材上的塗覆量和形態與光電流響應強度有密切關聯性。最後，以SDS流場對晶片上的PM進行去脂質實驗，發現過程中會有BR去褶疊與再褶疊及PM膜重整的情形發生，而使晶片光電流響應可被回復30%左右，顯示PM晶片的高穩定性於未來染料敏化電池及生物晶片的開發具有極大潛能。

Purple membrane (PM) is part of the cell membrane of archaeon Halobacterium salinarum, where bacteriorhodopsin (BR) resides as the major protein and acts as a light-driven proton pump. In this thesis, self-assembled monolayers (SAM) formed by carboxyalkylphosphonic acids were employed to fabricate ITO glass, followed by amination to subsequently immobilize PM through bioaffinity interactions. The layer-by-layer coating was confirmed by both contact angle and cyclic voltammetry measurements, with the observation of variation of contact angles from 3~5° on ITO, up around 75° on the SAM layer, and finally down to 20~30° on PM, and of decrease of redox peak-currents of the electrode with increased coating layers. The coating was also confirmed by XPS analyses. The photocurrent responses of the PM-coated chips strongly depended on the buffers to suspend PM and to post-wash chips and on the pHs of the measuring electrolyte, with the achievement of a maximum photocurrent density of 1.1±0.1 μA/cm2. Both AFM and XPS analyses suggested the coating amount and morphology of PM on ITO glass significantly affected the photoelectric performance of the prepared chips. Finally, delipidation of the immobilized PM with a SDS flow resulted in BR unfolding and refolding and in PM displacement and reorganization. About 30% of the chip-photocurrent activities were recovered, demonstrating the robustness of the prepared PM-coated chips and suggesting the PM potential for future applications in DSSC and biochips.

余安棣，"製備具高度方向性Bacteriorhodopsin生物光電晶片"，國立台灣科技大學化學工程研究所碩士論文 (2009)

陳逸航，"定向性細菌視紫質晶片之光電與二倍頻響應探討"，國立台灣科技大學化學工程研究所碩士論文 (2010)

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