本論文結合了奈米合成技術與生物醫學應用，將低毒性之量子點應用於細胞及生物活體上，成功開發出具有低成本且合成時間短、高量子效率、低毒性、螢光顯影標靶、核磁共振之多功能量子點材料。
第一部分：以水為溶劑，使用微波輔助法成功地直接合成出水溶性Ag+、Mn2+離子共摻雜In2S3量子點 (簡稱Ag/Mn:In2S3)。藉由優化前驅物比例以改善材料缺陷性質，並使用雙穩定劑L-glutathione與Citric acid trisodium salt讓量子點能夠在水中穩定地合成。後續再將此量子點以無機材料ZnS包覆而形成Ag/Mn:In2S3/ZnS核殼型量子點，具備45.24%螢光量子產率。最後利用TEM、XRD、UV、PL、EPR、ICP、FTIR及Life time來進行摻雜量子點的組成、結構、光學性質與磁性分析。結果發現Mn2+離子螯合於In2S3量子點之中使其展現出順磁特性; 而Ag+離子摻雜In2S3量子點改變其光學能隙值，使量子點具有可調性放光波長特性 (472 nm~743 nm, 綠光至近紅外光)。
第二部分：為增添其生醫應用性，利用EDC/Sulfo-NHS共價交聯反應將Ag/Mn:In2S3/ZnS量子點表面修飾玻尿酸(hyaluronic acid, HA)，使此量子點具有專一性標靶的功能，能夠針對特定的癌細胞進行有效的顯影以及追蹤。癌細胞和生物活體的斑馬魚毒性實驗，也證明Ag/Mn:In2S3/ZnS量子點表面功能化後，依舊為無毒奈米生醫材料。在細胞影像中，證明Ag/Mn:In2S3/ZnS@HA量子點兼具螢光標靶顯影與核磁共振標靶顯影之雙重顯影效果。

We present a facile microwave-assisted method successfully direct synthesis of water-soluble Ag+ and Mn2+ co-doped In2S3 quantum dots (Ag/Mn:In2S3 QDs) with glutathione (GSH) and citric acid trisodium salt (SC) as the dual stabilizing agents. ZnS semiconducting material was designed to passivate on the surface of Ag/Mn:In2S3, leading to the formation of Ag/Mn:In2S3/ZnS core/shell QDs with quantum yields up to 45.24%. The as-prepared Ag/Mn:In2S3/ZnS QDs exhibited small particle sizes (~2.5 nm), long photoluminescence lifetime, and color-tunable properties ranging from 472 to 743 nm by tailoring the stoichiometric ratio of [Ag]/[In].
To demonstrate their potential biomedical application, hyaluronic acid (HA) was used to conjugate with Ag/Mn:In2S3/ZnS core/shell QDs using EDC/Sulfo-NHS chemistry. Confocal imaging characterization revealed that functionalized Ag/Mn:In2S3/ZnS@HA QDs could be specifically targeting and effectively uptaked by B16F1 cells. In vivo imaging confirmed that Ag/Mn:In2S3/ZnS@HA QDs could be a dual-modal magnetic resonance and fluorescence imaging. Finally, zebrafish experiments also proved that Ag/Mn:In2S3/ZnS@HA QDs remains non-toxic features after surface functionalization.

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