研究生: |
Dinh Thi My Huong Dinh Thi My Huong |
---|---|
論文名稱: |
固定在醋酸纖維素和纖維素為基材的奈米纖維膜上的聚六亞甲基雙胍的抗菌活性研究:使用和不使用反應性綠 19 染料作為偶聯劑 A study on the antibacterial activity of poly(hexamethylene biguanide) immobilized on cellulose acetate and cellulose-based nanofiber membranes: With and without reactive green 19 dye as a coupling reagent |
指導教授: |
蔡伸隆
Shen-Long Tsai 張煜光 Yu-Kaung Chang |
口試委員: |
張煜光
Yu-Kaung Chang 王勝仕 Sheng-Shih Wang 藍祺偉 Chi-Wei Lan 李振綱 Cheng-Kang Lee 蔡伸隆 Shen-Long Tsai |
學位類別: |
博士 Doctor |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2023 |
畢業學年度: | 112 |
語文別: | 英文 |
論文頁數: | 126 |
中文關鍵詞: | 奈米纖維膜 、醋酸纖維素 、再生纖維素 、聚六亞甲基雙胍 、抗菌活性 、大腸桿菌 、反應性 染料 |
外文關鍵詞: | Nanofiber membrane, Cellulose acetate, Regenerated cellulose, Poly(hexamethylene biguanide), Antibacterial activity, E.coli, Reactive dye |
相關次數: | 點閱:342 下載:0 |
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廣泛研究了固定聚六亞甲基雙胍(PHMB)未染色/染色醋酸纖維素及纖維素奈米纖維膜的抗菌性能。 這項研究使用靜電紡絲生產醋酸纖維素 (CA) 奈米纖維膜,在鹼性溶液中水解形成 再生纖維素(RC) 奈米纖維膜。 然後將兩種類型的奈米纖維膜與 PHMB 固定以生產 CA-PHMB 和 RC-PHMB 奈米纖維膜。 結果表明,固定PHMB使奈米纖維膜的抗菌功效(AE,%)從CA-PHMB奈米纖維膜的65.67%增加到86.13%,將RC-PHMB奈米纖維膜從35.09%增加到100%。 根據化學和物理特性,抗菌功效受到 CA 奈米纖維去乙醯化、表面電荷和 PHMB 密度的顯著影響。 RC-PHMB 奈米纖維膜在大腸桿菌作用 10 分鐘後表現出 100% AE。 此外,即使在儲存20天後,RC-PHMB奈米纖維膜的AE仍保持在100%左右,證明了固定在RC奈米纖維膜上的PHMB的穩定性。
染色抗菌膜 CA-RG19-PHMB 和 RC-RG19-PHMB 奈米纖維膜是透過反應性綠 19 染料 (RG19) 處理 CA 和 RC 奈米纖維膜,然後用 PHMB 固定它們來增強抗菌活性而製成的。CA-RG19-PHMB 奈米纖維膜在 5 mg/ml RG19處理3小時,0.004 (mg/ml) PHMB處理15分鐘,僅在 10 分鐘左右對大腸桿菌達100% AE。 RC-RG19-PHMB奈米纖維膜在1 mg/ml RG19處理3小時和0.003 (mg/ml) PHMB處理15分鐘,在2小時左右對大腸桿菌達100% AE 。 儲存 60 天後,兩種膜均保留 100% AE,並在五個測試週期後顯示相似的結果。 這些發現顯示CA-RG19-PHMB和RC-RG19-PHMB奈米纖維膜是有效且耐用的抗菌材料,適合各種應用。 總之,這些結果表明,新型RC-PHMB奈米纖維膜作為抗菌材料在生物醫學應用、食品包裝產業以及水過濾或處理方面具有廣闊的前景。
The antibacterial properties of undyed/dyed cellulose acetate/cellulose nanofiber membranes immobilized with poly(hexamethylene biguanide) were extensively studied. This study used electrospinning to produce cellulose acetate (CA) nanofiber membranes hydrolyzed in an alkaline solution to create regenerated cellulose (RC) nanofiber membranes. Both nanofiber membranes were immobilized with PHMB to produce CA-PHMB and RC-PHMB nanofiber membranes. Results revealed that immobilizing PHMB increased the antibacterial efficacy (AE, %) of the nanofiber membranes from 65.67% to 86.13% for CA-PHMB nanofiber membranes and from 35.09% to 100% for RC-PHMB nanofiber membranes. Antibacterial efficacy was significantly influenced by CA nanofiber deacetylation, surface charge, and PHMB density based on chemical and physical properties. RC-PHMB nanofiber membrane demonstrated 100% AE after 10 min with E. coli.
Furthermore, the AE of the RC-PHMB nanofiber membrane remained around 100% even after 20 days of storage, demonstrating the stability of PHMB immobilized on the RC nanofiber membrane. Colored antibacterial membranes, CA-RG19-PHMB and RC-RG19-PHMB nanofiber membranes, were created by treating CA and RC nanofiber membranes with reactive green 19 dye (RG19) before immobilizing them with PHMB to enhance antibacterial activity. CA-RG19-PHMB nanofiber membrane, treated with 5 mg/ml RG19 for 3 h and 0.004 (mg/ml) PHMB for 15 min, achieving 100% AE against E. coli in only 10 min. RC-RG19-PHMB nanofiber membrane, treated with 1 mg/ml RG19 for 3 h and 0.003 (mg/ml) PHMB for 15 min, achieving 100% AE against E. coli in 2 h. After 60 days of storage, both membranes retained 100% AE and displayed similar results after five testing cycles. These findings indicate that CA-RG19-PHMB and RC-RG19-PHMB nanofiber membranes are effective and durable antibacterial materials suitable for various applications. In conclusion, these findings suggest that the novel RC-PHMB nanofiber membranes hold great promise as antibacterial materials for biomedical applications, the food packaging industry, and water filtration or treatment.
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