研究生: |
曾逸群 Yi-Qun Tseng |
---|---|
論文名稱: |
金奈米粒子-氧化石墨烯-奈米纖維複合層析紙於表面增強拉曼光譜之應用 Gold Nanoparticles-Graphene Oxide-Cellulose Nanofibers based Chromatographic SERS Paper for Bio-Detection |
指導教授: |
楊銘乾
Ming-Chien Yang |
口試委員: |
楊銘乾
Ming-Chien Yang 劉定宇 Ting-Yu Liu 鄭詠馨 Yung-Hsin Cheng |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2019 |
畢業學年度: | 107 |
語文別: | 中文 |
論文頁數: | 81 |
中文關鍵詞: | 奈米纖維素 、氧化石墨烯 、金奈米粒子 、表面增強拉曼光譜 |
外文關鍵詞: | TOCNs, gold nanoparticles, graphene oxide, surface-enhanced Raman scattering (SERS) |
相關次數: | 點閱:490 下載:0 |
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本研究利用纖維素純化技術,純化針葉纖維素,以TEMPO (2,2,6,6-tetramethylepiperidin-1-oxyl)/NaBr/NaClO氧化纖維素中的羥基,引入羧基及醛基,將其纖維素奈米化,改質過後的奈米纖維 (TONCs),透過XRD繞射分析顯示TOCNs保有纖維素原本結晶度及完整性。在實驗中,我們添加氧化石墨烯(GO)於TOCNs中,GO特殊的苯環結構有助於增強拉曼光譜訊號,並能增加紙製品的機械強力(約7-8倍),製作出具高延展性之紙製品(GO@TOCNs)。隨後我們將金奈米粒子(AuNPs)導入GO@TOCNs複合紙表面,使AuNPs@GO@TOCNs複合紙具有表面增強拉曼(surface enhance Raman scattering, SERS)檢測能力及層析能力,藉由調控TOCNs及GO的比例,可將待測物有效層析分離。本次實驗之檢測分子為羅丹明6G (Rhodamine 6G)及直接藍(Direct blue),透過AuNPs@GO@TOCNs複合層析紙初步層析分離,再使用SERS技術來做檢測,其最低檢測濃度<10-7M,作為新興的生醫快篩檢測試紙,未來可用於民生、環保及生醫產業上(例如:水中污染物、生物分子、細菌檢測等)。
The nanohybrids of surface-enhanced Raman scattering (SERS) detection papers were successfully fabricated by gold nanoparticles (AuNPs) immobilized on the graphene oxide (GO) nanosheets-cellulose nanofibers (TOCNs) substrates. TOCNs were extracted from the recycling cellulose fibers from the food wastes, and then modified by a 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) to exfoliate it. GO and TOCNs were further blended together to fabricate papers by the paper machine. Furthermore, AuNPs were (ca. 10 nm) in-situ grown on the GO@TOCNs paper as SERS substrate for biomolecules and water pollutants detection. [2] The result show that the tensile strength of AuNPs@GO@TOCNs composites is 7-8 times higher than the pristine TOCNs papers, showing the great mechanical properties after the addition of GO and AuNPs. X-ray diffraction patterns show that TOCNs still maintain great crystallinity, even though diameters of cellulose fibers decrease from micro- to nano-scale. The biomolecules of rhodamine 6G and direct blue can be detected by Raman spectroscopy and chromatographic separation. The limit of detection (LOD) on AuNPs@GO@TOCNs SERS paper is lower than 10-7 M. The reason is that GO can doubly enhance the SERS intensity by the surface plasmon resonance in the symmetrical benzene structure. Finally, the nanohybrids paper can be used as a chromatographic paper to separate the samples by modulated the ratios of GO and TOCNs. Thus, AuNPs@GO@TOCNs papers are potential to apply in the platform of the chromatographic separation and rapid SERS detection of biomolecules.
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