玻尿酸（Hyaluronic acid）為一黏性多醣體，可利用微生物醱酵獲得，在醫藥美容方面應用範圍廣而具有高經濟價值，但其純化過程繁複造成生產成本增加，因此本研究主要目的為建立一個快速回收玻尿酸的系統以降低玻尿酸生產成本。
首先利用修飾完成，具正電性及疏水端的磁微粒吸附回收玻尿酸，探討在不同吸附條件下之最大吸附量。不同鹽濃度下之吸附情形說明親和配體（Ligand）與玻尿酸間可利用靜電作用吸附；不同溫度下之恆溫吸附結果證明磁微粒與玻尿酸間存在疏水作用力。在最佳吸附條件下，修飾過之磁微粒每克可回收7.72 mg的玻尿酸；應用在純化醱酵液中之玻尿酸，由於雜質非專一性吸附以及醱酵液中之鹽濃度而降低磁微粒吸附玻尿酸能力，每克磁微粒回收1.21 mg之玻尿酸。
根據抗菌實驗測試結果，選用的親和配體具有抑菌效果，能有效抑制S. zooepidemicus菌株生長情形。

Hyaluronic acid ( HA ) can be produced through microbial fermentation by using Group A and C streptococci. Traditionally, it is recovered and purified from the fermentation broth by adding Cetylpyridinium chloride (CPC) to form complex with HA. The complex forming effect between CPC and HA involve the hydrophobic and attractive electrostatic interaction. However, the HA recovered by the CPC complexing method may be contaminated with the fermentation media that lowers its purity. Besides, centrifugation or filtration has to be employed to recover the precipitated complex. Silica coated magnetite can be easily modified with a chemical 3-trimethoxy silyl propyl dimethyl octadecyl ammonium choloride (AGIS) which has very similar structure as CPC to become M-AGIS and employed to purify HA from fermentation broth.. By applying magnetic field of enough strength, M-AGIS can be totally recovered. The adsorption isotherm of HA to M-AGIS at pH 4, 37 0C can be described by Langmuir adsorption model with maximum adsorption capacity Qmax of 7.72 mg/g and dissociation constant Kd of 0.0165. Higher temperature favors the adsorption. HA can be eluted from the adsorbent M-AGIS with about 80 % yield by employing 1 M NaCl. By employing M-AGIS, the recovery and purification of HA directly from HA producing cells suspension has been demonstrated.

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