生醫光電是一新興且成長迅速的研究領域，近年來已成為生物科技之重點發展項目之一。由於醫療技術日益進步，使得人類平均壽命提高，間接也創造了健康照護的市場。尤其在醫療器材技術日新月異，先進國家重視個人健康概念，居家照護及遠距醫療等服務逐漸盛行等因素驅動下，光電感測產業更已成為生醫光電領域最耀眼的明星。在智慧穿戴裝置興起後，加上巨量資料分析技術的成熟，生理訊號監測系統與無線通訊設備的相結合，可望將各種個人生活習慣與醫學標準數據整合傳送至醫療機構，不但可提供個人化醫療服務，同時也可提供重要資訊，以改進醫療技術或保健成本效益，這也是光電感測產業未來性備受看好的主因。
生醫感測可分成侵入式與非侵入式兩種形式量測，侵入式包含血糖儀、肝炎檢測儀、基因、血液檢測儀等；非侵入式則涵蓋血壓、心跳、血氧、心電圖、體溫等檢測儀，檢測方式與光學技術關係密切。
表面電漿共振是一種存在於金屬與非導電介質界面的物理現象。當一束偏極光進入玻璃稜鏡時，會產生全反射。光在行進的時候，會與稜鏡上所覆蓋的金屬薄膜的原子產生共振，並產生衰逝波進入介電質層。當光經由稜鏡反射之後，在某一特定角度或波長的光強度會因為共振作用而急速下降，而光之相位則是會發生劇烈的變化，此一角度稱為共振角度，而波長稱為共振波長。改變介電質層之折射率時，共振角度或是波長會產生位移，相位則會產生變化，將此特性應用於生物感測器，具有即時檢測、高靈敏度與不須標記等特性，因此廣泛應用於生物檢測與免疫組織化學上，可有效的分析檢測物質之微小濃度折射率變化。常見的表面電漿共振量測方式有四種，角度調制、波長調制、強度調制與相位調制，其中又以相位調制之靈敏度為最高，然而自Nelson 等人(Nelson et al., 1996)提出相位量測方式以來，相關的研究似乎僅止於偵測極限的探討，忽略了動態量測的優勢，因此本論文便是利用自行建構的表面電漿共振相位感測儀，藉由測量反射光相位變化的方式，對流感DNA與其探針(probe)間的結合反應進行動態量測，達到其計算其反應速率常數目的，另外更深入探討如何優化流感檢體的探針(probe)，使其在與檢體鍵結有更好的效果。
計算流感檢體與其探針的速率常數結果顯示，用們自行建構表面電漿共振相位感測儀在1550nm波段所量測的結合速率常數9759.72M-1s-1，解離速率常數為0.0438s-1在，相較於Bicore3000在波長690nm所量測的結合速率常數47188.9 s-1和解離常數0.002342 s-1，本系統所測得速率常數較低，可能因為樣品溶液在管線中流動距離過長導致液體濃度遭到稀釋和液體輸送速率並沒有保持固定，同時相位變化的非線性趨勢，皆可能影響到最後速率常數的結果。而在優化固定化(Immobilization)方面，將流感DNA探針在固定化前，進行二硫鍵的還原，並利用胸腺嘧啶(T)組成的五組密碼子墊高原本探針高度，使的探針和流感DNA之間鍵結效果更加，最後量測到靈敏度為0.149rad/μg/ml。

Bioelectronics, one of the key developments in biotechnology, is a new and rapid research field. The mature medical care increases human life and also benefits the health care market. The related medical equipment gets significantly improved, and moreover the health concept is deeply pursued in the well-developed countries. Optoelectronics sensing industry then gets a lot of attentions in the field of biomedical care. After an intelligent wear device into the market, the large amount of data transmission regarding a variety of personal health monitoring signals will not only provide personalized medical services, but also supply important information to the medical analysis for human health benefits.
There are two kinds of biosensor, invasive and non-invasive. The invasive approach includes the glucose meter, the detector for hepatitis, gene and blood. The non-invasive type covers blood pressure, heart beat rate, blood oxygen, ECG, and body temperature detection.
Surface plasmon resonance (SPR) is a physical phenomenon that happens between the interface of metal and non-conductive material. When a polarized light wave is entering a glass prism and satisfying the total internal reflection, the incident light will resonate with the thin metal film which is covered on the prism and generate the evanescent wave that penetrates into the dielectric layer. When the light is reflected by the prism at a specific angle or wavelength, the optical power will rapidly drop to minimum because of the resonant effect. In the meantime, the reflected light phase will also change dramatically. This specific angle and wavelength are named resonant angle and resonant wavelength, respectively. When the refractive index of a dielectric layer is changed, the resonant angle or resonant wavelength will shift, same as the reflected light phase. By applying these features onto biosensors, the real-time, high sensitivity and label-free detection could be demonstrated. Therefore, it has been extensively utilized in bio-sensing and immunochemistry for its efficiency in analyzing the small refractive index variation of detected materials. Among four common SPR modulation approaches, angle modulation, wavelength modulation, Intensity modulation and phase modulation, the phase modulation demonstrates the highest sensitivity. Since the phase measurement method was proposed for characterization, it mainly focused on improving detection limit and other bio-applications, lack of the dynamic analysis in bio molecular interactions. Here a homemade phase-based SPR system will be demonstrated to derive the kinetic parameters from Avian Influenza DNA SPR sensing. Moreover, the Avian influenza DNA probe immobilization will be also optimized for better sensitivity.
Finally, we conducted the dynamically binding test for Avian Influenza DNA and its probe using both the homemade SPR system and use a commercial Biacore3000 SPR system for comparison. The association (ka) and dissociation (kd) rate constants obtained from the homemade system were 9759.72M-1s-1and 0.0438s-1. Compared with the Biacore3000 SPR system, we found that the ka value obtained from the home-made system was smaller than that the Biacore3000. This discrepancy was mainly from the dilution effect, in which the adjacent running buffer diluted the sample solution. The other issue may come from the nonlinear phase shift. In the immobilization optimization, the influenza DNA probe will be treated using the disulfide bond reduction to increase the original probe height for efficiently sense the influenza DNA. Finally, the sensitivity was 0.149 rad/μg/mL .

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