近年來因腫瘤的盛行率，而導致死亡率的增加。因腫瘤分為幾個不同的階段，會產生不同程度的血流影響效應，因此本研究藉由建構血液循環功能整合評估系統，來探討血流相關參數與腫瘤的相關性。
實驗設計方面採用雷射都卜勒血流量測技術對局部血流之偵測具有高敏感度，由於它的光學纖維探頭輕薄短小、不具侵犯性且又不會對正常組織內的微循環生理狀況產生干擾，也可以應用活體研究中進行連續性的偵測與分析。本實驗的對象為70隻裸鼠，將其分為空白組、沒餵藥組、餵Statin組、餵Metformin組與同時餵兩種藥，後面四組都將植入CT26的癌細胞。在這裡分為三大部分來探討，分別為空白組與腫瘤組的比較(由LDF各血流參數的變化來評估是否有腫瘤)、腫瘤的轉移與否比較(由LDF早期各血流參數的變化來預估是否有腫瘤的轉移)與餵食不同藥物的比較(藉由每天餵食不同藥物去監控各血流參數的改變)，希望能從中看出不同的差異存在。
本實驗使用MATLAB軟體針對檔案進行資料的處理與分析，配合由實驗室獨立開發出之時域波型參數與時域變異率參數以及頻域之各血流參數進行比較，並利用生物統計方式去分析腫瘤對血管特性的影響程度，經由比對可看出其有明顯差異(P<0.05)。
從實驗成果可瞭解惡性腫瘤對血流相關參數的變化有所不同，也能從數據走向得知不同餵藥的影響程度，甚至可以從數據看出轉移與否。未來如能從數據趨勢預測受測者是否會罹患癌症，將有助於早期偵測與早期治療。

In recent years, due to the prevalence of the tumor, which leads to increased high mortality. Tumor is divided into several different stages, which cause varying degrees of blood flow effect. Therefore, our study explores correlation between blood flow parameters and tumor by constructing integrated assessment of blood circulation system.
Design of experiments using laser Doppler blood flow measurement techniques to detect local blood flow that it has high sensitivity, since the optical fiber probe is slim and light, non-invasive and because they are not on microcirculation physiology in the normal organization interference, also can be applied to continuous detection and analysis performed in vivo studies. The object of the experiment was 70 nude mices were divided into control group, no feed drugs group, feed Statin group, feed Metformin group and simultaneously feed two drugs, behind the four groups will be implanted CT26 cancer cells. Here are three parts to the discussion, respectively compare control group and tumor group (all kinds of hemodynamic parameters change to assess whether there is a tumor by LDF), tumor metastasis or not compared (all kinds of early hemodynamic parameters change to estimate whether there is metastasis), and comparison of feed different drugs (by feeding different drugs every day to monitor change of hemodynamic parameters), hoping to see the various differences.
Our experiment analyzes data by MATLAB software and our developed testing system including time domain waveform parameters, time domain variation rate parameters, and the hemodynamic parameters. We not only compare these parameters in detail, but also use statistical methods to analyze biological effects on tumor vascular characteristics. Our result shows significant difference by our experiments (P<0.005).
Experiments show that malignant tumor changes as related blood parameters goes by and, the different degree of affects influenced by various medicines. If we can predict whether someone suffers cancer or not by data in advance, it will help in the early detection and early treatment.

[1]Arthur C. Guyton and John E. Hall 著, 賴亮全, 林則彬 與 林富美 合譯, Human Physiology and Mechanisms of Disease (Sixth edition), Farseeing Publishing Co., Ltd., pp. 182 (2004)
[2]行政院衛生福利部國民健康署, "中華民國103年死因統計," ed, 2014.
[3]Pin-Tsun Chao, Wen-Rei Chiang, Chia-Liang Hsu, Ming-Yie Jan, Wei-Kung Wang, and Yuh-Ying Lin Wang, “Effect of Pulse Number on the Pulse-Based Synchronized-Averaging Analysis in Laser doppler Signal,” Biomedical Engineering: Applications, Basis and Communications(BME), Vol. 19, No. 3, pp. 179-185, 2007
[4] LDF取自網址：http://www.labmed.org.tw/publish_1.ASP?mno=244
[5]A. P. Shepherd and P. Å. Öberg, Laser-Doppler blood flowmetry vol. 107: Springer, 1990.
[6]P T Chao, M Y Jan, H Hsiu, T L Hsu,W K Wang, and Y Y Lin Wang, “Evaluating microcirculation by pulsatile laser Doppler signal,” Physics in Medicine and Biology, Vol. 51, pp. 845–854, 2006
[7] Yih-Kuen Jan, Bryan D. Struck, Robert D. Foreman ,and Cyndy Robinson, “Wavelet analysis of sacral skin blood flow oscillations to assess soft tissue viability in older adults,” Elseviver: Microvascular Research, Vol. 78 pp.168-168 (2009)
[8]裸鼠 取自網址: http://baike.baidu.com/view/1257286.htm
[9]血管新生 取自網址:
http://www2.cmu.edu.tw/~cmcrdc/epaper/detail.php?epaperno=23&class=C&id=148
[10]CT26取自網址: http://baike.baidu.com/view/9707024.htm
[11]Statin取自網址:http://www.tma.tw/magazine/ShowRepID.asp?rep_id=1497
[12]Metformin取自網址: http://translate.google.com.tw/translate?hl=zh-TW&sl=en&u=https://en.wikipedia.org/wiki/Metformin&prev=search
 
[13]Pin-Tsun Chao, Wen-Rei Chiang, Chia-Liang Hsu, Ming-Yie Jan, Wei-Kung Wang, and Yuh-Ying Lin Wang, “Effect of Pulse Number on the Pulse-Based Synchronized-Averaging Analysis in Laser doppler Signal,” Biomedical Engineering: Applications, Basis and Communications(BME), Vol. 19, No. 3, pp. 179-185, 2007
[14]P T Chao, M Y Jan, H Hsiu, T L Hsu,W K Wang, and Y Y Lin Wang, “Evaluating microcirculation by pulsatile laser Doppler signal,” Physics in Medicine and Biology, Vol. 51, pp. 845–854, 2006
[15] Tse Lin Hsu, Pin Tsun Chao, Ming Yie Jan, Wei Kung Wang b, Sai Ping Li, and Yuh Ying Lin Wang, “Effects of Angiotensin II on Flux Rise Time in Rats (A Time Index of Laser Doppler Flowmetry) and its Relation with Microvascular Structures,”Elseviver: Microvascular Research, Vol. 75, pp. 211–216, 2008
[16]Aneta Stefanovska, Maja Bracic, and Hebe D´esir´ee Kvernmo, “Wavelet Analysis of Oscillations in the Peripheral Blood Circulation Measured by Laser Doppler Technique, ”Institute of Electrical and Electronics Engineers: Transactions on Biomedical Engineering, Vol. 46, No. 10 (1999)
[17]楊舒涵, ”都卜勒微循環血流與動脈血壓波形指標應用於代謝症候群之研究,” 碩士論文, 國立台灣科技大學, 台北, 201
[18]吳冠賢, ”雷射都卜勒血流時域與頻域分析應用於糖尿病之指標開發研究,” 碩士論文, 國立台灣科技大學, 台北, 2012
[19]Tumor Endothelial Cells Andrew C. Dudley The Department of Cellular and Molecular Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599; Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina 27599; and McAllister Heart Institute, Chapel Hill, North Carolina 27599
[20]Blood Pressure and Risk of Cancer Incidence and Mortality in the Metabolic Syndrome and Cancer Project Tanja Stocks, Mieke Van Hemelrijck, Jonas Manjer, Tone Bjrge, Hanno Ulmer, Göran Hallmans, Björn Lindkvist, Randi Selmer, Gabriele Nagel, Steinar Tretli, Hans Concin, Anders Engeland, Håkan Jonsson, Pär Stattin