研究生: |
黃昱瑄 Yu-Hsuan Huang |
---|---|
論文名稱: |
包覆阿黴素之聚乳酸單孔空心微球製備與其作為藥物載體之研究 Preparation of doxorubicin loaded polylactide hollow microspheres and their application in drug delivery system |
指導教授: |
白孟宜
Meng-Yi Bai |
口試委員: |
鄭詠馨
Yung-Hsin Cheng 王毓淇 Yu-Chi Wang |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 醫學工程研究所 Graduate Institute of Biomedical Engineering |
論文出版年: | 2022 |
畢業學年度: | 110 |
語文別: | 中文 |
論文頁數: | 96 |
中文關鍵詞: | 空心微球載體 、聚乳酸 、乳腺癌細胞 、阿黴素 、溶劑蒸發法 、乳化法 |
外文關鍵詞: | Hollow microspheres carrier, Poly(L-lactic acid), Breast cancer cells, Doxorubicin, Solvent evaporation, Emulsification |
相關次數: | 點閱:247 下載:0 |
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藥物載體會根據不同應用使用不同製備方式而有不同的結構,如實心球體、中空球體、多孔球體、單孔球體、殼核球體等。其中最常見的製備方式是乳化法,它是利用乳化劑將高分子油相溶於水相中,乳化劑是作為穩定並讓兩相共存的界面活性劑。乳化劑的濃度會直接影響到兩相的穩定性以及微球的粒徑大小,而不同的高分子溶劑會直接影響到微球表面的粗糙度等。生物可降解的高分子,如PLA、PCL、PLGA等,由於其降解特性可穩定緩慢釋放藥物,長時間對癌細胞造成傷害,因此成為現今作為藥物載體材料的首要選擇。有文獻指出結晶高分子載體可以利用材料熱力學之特性使其自行將孔洞密合。本篇研究是將聚左旋乳酸以乳化法配合溶劑蒸發法一步製備出單孔空心微球載體,粒徑可小至6-9微米,並利用材料本身熱力學特性以簡單的方式包覆阿黴素作為藥物載體並對小鼠乳腺癌細胞JC (CRL-2116)進行細胞毒性測試,在24小時藥物釋放後對癌細胞達到約48%的殺滅效果。
According to applications, carriers is prepared by different methods to product many kinds of structures, such as solid, hollow, porous, single-hole, shell-core, etc. Emulsification is the most common method of product drug carrier, which uses an emulsifier to dissolve and stabilize the both of oil and water phase. The stability of emulsion and the particle sizes are directly affected by the concentration of the emulsifier, and the surface roughness of polymer microspheres are directly affected by the solvents for the polymer. Biodegradable polymers, such as PLA, PCL, PLGA, etc., are the best choice for medical materials currently, because of its degradation characteristics, the drug release will be continuously and steadily that lethal to cancer cells for a long time. The literatures point out that crystalline polymer can be use of their thermodynamics properties to make the pores closing by itself. In this study, we prepare of poly (L-lactic acid) hollow microspheres carrier by an emulsification and a solvent evaporation method, the particle size can be as small as 6-9 um. Using the traditional cancer treatment drug, doxorubicin was loaded in a simple way by the thermodynamic properties of PLLA, and was used for mouse breast cancer cells JC (CRL-2116) to test for cytotoxicity. It achieved approximately 48% killing effect on JC cells after 24 hours of drug release.
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