研究生: |
Addisu Getachew Destaye Addisu - Getachew Destaye |
---|---|
論文名稱: |
Antimicrobial Electrospun Polyvinyl Alcohol (PVA) Nanofibrous Mat with Incorporating Glucose Oxidase, Silve Nanoparticles, and N-Halamines Modification Antimicrobial Electrospun Polyvinyl Alcohol (PVA) Nanofibrous Mat with Incorporating Glucose Oxidase, Silve Nanoparticles, and N-Halamines Modification |
指導教授: |
李振綱
Cheng-Kang Lee |
口試委員: |
今榮東洋子
Toyoko Imae 王孟菊 Meng-Jiy Wang Christopher Whiteley Christopher Whiteley 劉懷勝 Hwai-Shen Liu 王勝仕 Steven S.-S. Wang |
學位類別: |
博士 Doctor |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2015 |
畢業學年度: | 103 |
語文別: | 英文 |
論文頁數: | 132 |
外文關鍵詞: | Nanofibrous mat, Polyvinyl alcohol, N-halamines, Vapor cross-linking, Epsilon polylysine |
相關次數: | 點閱:314 下載:1 |
分享至: |
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Nanomaterials are at the forefront of rapidly growing field of nanotechnology. Due to their nanoscale size, nanomaterials possess extremely high surface area to volume ratio. In this dissertation, antimicrobial nanofibers were made from electrospun polyvinyl alcohol (PVA) solutions. PVA is a very hydrophilic, biocompatible, and non-toxic synthetic polymer with excellent chemical, thermal, and mechanical properties that can easily be electrospun in to PVA nanofibrous mats. While these properties are desirable properties for encapsulating biological active proteins for various application, its further use in aqueous environment is limited by its solubility. Hence, in this study, PVA nanofibrous mats consisted of nanofibers (200 – 400 nm in diameter) were prepared via electrospinning and subjected to glutaraldehyde (GA: 0.5, 1.0, 2.0, and 2.56 M) vapor phase cross-linking at room temperature. The cross-linking not only resulted in a water-insoluble nanofibrous mats but also generated an excess amount of unreacted aldehyde functional groups (2.16 nmol/mm2 and 2.24 nmol/mm2) that were further used in in-situ reduction of silver salts in to silver nanoparticles (4.60 % for 2.0 M GA and 5.76 % for 2.56 M GA cross-linked mats) with average particle size of 20-46 nm. The unreacted end of the aldehyde groups (247 μmole/mm2) were also used for grafting nitrogen containing functional groups such as ε-polylysine that was then transformed in to rechargeable (97% rechargeability) N-halamines by bleaching (10% vol NaOCl). Glucose oxidase (GOx: 2.0%) and glucose (Glu: 1, 3, and 5.35 mg/mL) were also separately encapsulated via simultaneous electrospinning of PVA/GOx and PVA/Glu (4:1 volume ratio) dopes to form a self-sustained and capable of killing catalase positive bacteria PVA nanofibrous mat. The antimicrobial activity of the mat resulted from the hydrogen peroxide (H2O2) (150 μM) generated by reacting glucose with GOx. All the PVA nanofibrous mats prepared have shown excellent antimicrobial activity against both Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) bacteria and have the ability to kill more than 99% of the bacteria. Therefore, these nanofibrous materials may have potential applications as versatile antimicrobial materials in the field of health, food, biomedical industries, and textile.
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