本研究探討金屬離子(Fe3+, Cr3+, Cu2+, Ni2+, Co2+)與配体(甘氨酸，甘氨酸-甘氨酸，甘氨酸-甘氨酸-甘氨酸，L-纈氨酸，阿魏酸及沒食子酸)間之平衡常數。 利用酸鹼值-電位滴定在298.15K及0.15 mol‧dm-3 of NaNO3離子強度下探討此配体之質子化常數以及二成份與三成份金屬-配体錯合物之形成。 根據電位滴定數據，利用軟体(HYPERQUAD 2008)得到每一系統之錯合模式。Gibbs反應自由能(rG)以及Gaussian模擬程式被用來証實配体提供之結合位置以及預測由一金屬離子、一配体所形成錯合物之結構。
本研究討論每一系統所發生之現象。對金屬離子而言，穩定常數以下列趨勢降低： Fe3+ > Cr3+ > Cu2+ > Ni2+ > Co2+；此趨勢受電價影響且遵循Irving-William 二價金屬璃子定律。對配体而言，結構体積較小之配体 (甘氨酸)及較負電荷之配体(沒食子酸)所形成之錯合物較穩定。而對甘氨酸寡肽來說，肽骨幹上之氮當其以去質子態存在時可加強錯合物 之穩定性。在三成份系統中，含沒食子酸所形成錯合物之穩定性大於含 阿魏酸之穩定性。本研究並利用∆logK 及 logX 定量化比較混合配体錯合物與二成份錯合物之穩定性。.

The equilibrium constants for metal ions (Fe3+, Cr3+, Cu2+, Ni2+, Co2+) with ligands (glycine, glycylglycine, glycylglycyglycine, L-norvaline, ferulic acid and gallic acid) were reported in this work. The protonation constants of these ligands along with the formation of metal-ligand complex in binary and ternary systems were studied by using pH-potentiometric titration in aqueous solution at 298.15K and an ionic strength 0.15 mol‧dm-3 of NaNO3. The complexation model for each system was refined by software program “HYPERQUAD 2008” from potentiometric titration data. Moreover, Gibbs free energy of reaction (rG) that obtained from Gaussian modeling program were used to verify the contributing binding site of the ligands and to predict the structure of the one metal and one ligand complexes.
The phenomenon that occurred in each system was discussed. In terms of metal ion, the trend of stability constant decreases in the following order Fe3+ > Cr3+ > Cu2+ > Ni2+ > Co2+, which is affected by its electrical charge and following Irving-William rule for divalent metal ion. In terms of the ligand, the system with less bulky structure (glycine) and more negatively charged ligand (gallic acid) formed more stable complex than the other ligands. While for system with gycine oligopeptide, nitrogen in peptide backbone enhanced the complex stability while it existed in deprotonated form. In ternary system that contains gallic acid, the stability constant of the complexes are more stable than the systems with ferulic acid. The stability of mixed ligand complexes was quantitatively compared with corresponding binary complexes in term of ∆logK and logX.

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