自身免疫系統的傷口復原機制，係藉由嗜中性白血球產生活性氧物質(Reactive oxygen Species; ROS)，抑制傷口處之細菌生長並防止細菌感染，因此本論文以靜電紡絲技術以製備具產生抑菌能力之過氧化氫(Hydrogen Peroxide; H2O2)之傷口敷料，本論文之研究由兩個方向進行，第一種方式以葡萄糖氧化酶(Glucose Oxidase)與過氧化氫酶(Catalase)以體積比4:1比例下與聚乙烯醇(PVA)均勻混和並以外加電壓13.0-14.5 kV、工作距離為110 mm之條件下以電紡雙針頭方式將酵素同時分別包覆於奈米纖維中其纖維直徑150-250 nm，所形成之奈米纖維膜中葡萄糖氧化酶與過氧化氫酶活性分別為12.46±0.03 10-2U/cm2、67.38±4.65 U/cm2，於定面積0.785cm2提供1ml基質葡萄糖5 mg/ml的濃度，會產生8.94±0.74 mM過氧化氫，對大腸桿菌(Escherichia coli)以及金黃葡萄球(Staphylococcus aureus)菌皆有明顯之抑菌效果，其抑菌效率分別為77.07%與46.28%，所產生之過氧化氫亦會經過氧化氫酶的催化反應而產生氧氣。由於聚乙烯醇為水溶性高分子，因此為了增加其奈米纖維於水中之穩定性，藉著以戊二醛交聯以及市售Opsite Spray 噴霧塗佈兩種方式加以修飾。為了實際應用實驗中將纖維薄膜放置於4C條件下以維持酵素活性。第二種方式以高分子聚乙烯咯烷酮(PVP)與過氧化氫以體積比4:1下所形成之穩定錯合物以雙針頭電紡方式與過氧化氫酶聚乙烯醇纖維同時形成複合纖維薄膜，不須基質下即可釋放產生濃度為2.42±0.1 mM的過氧化氫，一般由於過氧化氫的不穩定性，容易裂解為水與氧氣，故將複合薄膜儲存於-20C下可維持95%的過氧化氫含量，此兩種方式所製備之薄膜因具備抑菌與產氧之特性，可應用於傷口敷料上使用。

During the wound healing process, the neutrophil can produce the reactive oxygen species (ROS) to kill the bacteria and prevent the infection. A nanofibrous mat with antimicrobial activity was produced by electrospinning. Glucose Oxidase (GOx) (950 U/ml) and Catalase (25 mg/ml) were incorporated in polyvinyalcohol (PVA) nanofibers by mixing enzyme into PVA solution in the volume ratio of 1:4. Both enzymes were encapsulated in the nanofibrous mat (150-250 nm fiber diameter) by simultaneous electrospinning using two separate needles at voltage 13.0-14.5 kV and 110 mm tip-to-collector distance. The activity of GOx and catalase in nanofibrous mats is 12.46±0.0310-2 U/cm2 and 67.38±4.65 U/cm2, respectively. By providing 1 ml of 5 mg/ml glucose to 0.785 cm2 nanofibrous mats, hydrogen peroxide (H2O2) will be generated first from the mat and the antibacterial activity was observed by measuring the inhibition zones of Escherichia coli and Staphylococcus aureus plates. H2O2 that produced from the GOx nanofibers can be catalyzed to generate oxygen (O2) by the catalase nanofibers of the mat. As PVA is water-soluble polymer, PVA nanofibrous mats stability required to be improved by GA cross-link and spray coating. For further application, the nanofibrous mats was stored at 4C to keep the activity of enzyme. Polyvinylpyrrolidone (PVP)-H2O2 complex was co-electrospun with PVA-Catalase nanofibers into a composite mat. without substrate, the nanofibrous mat itself can release H2O2 and show the antibacterial activity. H2O2 can be easily decomposed into water and oxygen, therefore by storing composite nanofibrous mats at -20C can maintain 95% of H2O2 amount. Two kinds of membrane prepared in this research is expected to be applied for wound dressing.

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