本論文主要由兩部分組成，第一部分論文之研究目標為製備一次性重金屬感測器，所採取的方式為利用靜電紡絲製備氧化鋁奈米纖維，並利用氧化鋁-聚苯胺複合材料修飾於可拋棄式的工作電極表面，以提升電極對重金屬感測之靈敏度，之後將修飾後的電極應用於感測鋅離子與銅離子。
為了增加電極對感測重金屬的靈敏度，本研究以化學合成方式製備具有導電性之聚苯胺高分子，並與電紡所製備的氧化鋁奈米纖維，以不同的體積比例均勻混合，接著利用減弱全反射-傅立葉轉換紅外線光譜儀 (ATR-FTIR)、X光繞射儀 (XRD)、場發射掃描式電子顯微鏡 (FE-SEM)等儀器，分析複合材料之化學組成、結晶型態以表面型態。本研究製備氧化鋁-聚苯胺複合材料，並修飾於可拋棄式碳電極 (screen printed electrode, SPE)工作表面，利用方波陽極溶出伏安法 (square wave anodic stripping voltammetry, SWASV)分別感測鋅離子與銅離子。結果顯示，所製備的電極感測鋅離子濃度時，感測靈敏度為1.45 uA ppb-1 cm-2 (線性範圍為80-200 ppb)，偵測極限為24.33 ppb。另外，同樣以方波陽極溶出伏安法感測銅離子，感測靈敏度為1.50 uA ppb-1 cm-2 (線性範圍為20-200 ppb)，偵測極限為7.70 ppb。接著，本研究同樣以氧化鋁-聚苯胺複合材料，修飾於可拋棄式金電極(gold electrode, AuE)工作表面，並同樣以方波陽極溶出伏安法感測銅離子，結果顯示感測靈敏度為6.54 uA ppb-1 cm-2 (線性範圍為20-200 ppb)，偵測極限為2.14 ppb。
本論文的第二部分，製備一次性DNA感測器，為提升DNA的固定效果，嘗試修飾三種不同含有胺官能基之材料：聚苯胺、聚乙烯亞胺、以及3-氨基丙基三乙氧基矽烷於電極工作表面，再以滴附法將probe DNA滴於電極上，接著同樣以滴附法將target DNA滴於電極工作表面，最後感測固定濃度之亞甲基藍，觀察在不同濃度之目標DNA下電流響應之差異，計算所製備電極對DNA之感測靈敏度以及其偵測極限。
本研究將probe DNA修飾於三種不同含有胺官能基改質的網印碳電極：PANI3/SPE、PEI3/SPE、APTES/SPE，以方波陽極溶出伏安法，感測固定濃度之亞甲基藍溶液。實驗結果發現在PEI3/SPE組別中，電極對target DNA感測靈敏度為0.65 uA uM-1 cm-2 (線性範圍為10-50 uM)，偵測極限為74.0 uM。然而在APTES/SPE組別中，電極對target DNA感測靈敏度為4.37 uA uM-1 cm-2 (線性範圍為1-10 uM)，高濃度時感測靈敏度為0.87 uA uM-1 cm-2 (線性範圍為10-50 uM)，偵測極限為4.83 uM。

In this study, two different electrochemical sensors were developed, aiming to be applied for onsite detection for metal ions and methylene blue, respectively. At the first part, the electrochemical sensors based on the anodic stripping voltammetry were developed for the detection of heavy metals, including zinc and copper ions. The aim of the second part of this study is to develop electrochemical sensors for DNA detection.
For first part of the thesis, two electrodes were applied for evaluating the sensitivity toward zinc and copper ions: (I) screen printed electrode (SPE), and (II) gold electrode (AuE). In order to enhance the sensitivity and the limit of detection (LOD), the electrode was modified with electrospun a-Al2O3 fibers mixed with polyaniline (PANI) (a-Al2O3-PANI). The functionalities, crystallinity, morphology of a-Al2O3 were characterized by ATR-FTIR, XRD, and FE-SEM, respectively. To detect zinc and copper ions by square wave anodic stripping voltammetry (SWASV). The a-Al2O3-PANI/SPE revealed a good sensitivity of 1.45 uA ppb-1 cm-2 toward zinc ions with the linear range of from 80 to 200 ppb, and the limit of detection of 24.33 ppb. On the other hand, the a-Al2O3-PANI/SPE and a-Al2O3-PANI/AuE revealed good sensitivity of 1.50 and 6.54 uA ppb-1 cm-2 toward Cu2+ ions, respectively. The linear range for the detection of Cu2+ was from 20-200 ppb on both electrodes, and the limit of detection was 7.70 and 2.14 ppb, respectively.
For the second part of the thesis, the surface of SPE was modified to prepare DNA sensor. In order to enhance the affinity between DNA and SPE, the electrode was modified with three different amine functionalities containing compounds: PANI, PEI, and APTES. The sensitivity and LOD (limit of detection) of the prepared sensors was determined toward methylene blue (MB). For the SPE decorated with PEI3(PEI3/SPE), the sensitivity for the detection of MB was 0.65 uA uM-1 cm-2,with the linear range from 10 to 50 uM, and the LOD of 74.0 uM. For the APTES/SPE, the sensitivity toward MB detection was 4.37 uA uM-1 cm-2 at lower concentration range from 1 to 10 uM. On the other hand, the sensitivity was 0.87 uA uM-1 cm-2 at higher concentration, from 10 to 50 uM, with the LOD of 4.83 uM.

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