作為應用於骨組織工程上的材料，除了生物相容性之外，骨誘導性（Osteoinduction）為材料所需具備的一項重要的性質，在本研究中利用模擬人體體液（Simulated body fluid，SBF）來披覆仿生礦化的氫氧基磷灰石於幾丁聚醣/動物明膠支架上，期望利用此仿生礦化層來引導骨細胞生長並誘導其分化以形成新生骨。經過ATR-FTIR分析可以發現氫氧基磷灰石中的磷酸根可與鈣離子形成磷酸鈣沈積，而SEM及X-ray繞射分析得知支架表面被覆著結晶性的圓球狀碳酸鹽類氫氧基磷灰石。體內動物實驗則是浸泡過類人體體液不同天數（3, 5, 7, 14及21 天）後的支架，將其植入於Wistar老鼠後腿肌肉內，於不同植入天數（7及14天）取出支架及周圍組織塊經冷凍切片後進行組織染色：蘇木紫-伊紅染色來檢驗支架之生物相容性，鹼性磷酸酶染色檢測鹼性磷酸酶表現，並輔以免疫組織染色：連骨質染色及骨涎蛋白染色分別檢測骨特異蛋白連骨質及骨涎蛋白之表現。實驗結果顯示出經過SBF處理的支架具有良好的生物相容性且可促進早期骨分化標記-鹼性磷酸酶表現，此外，浸泡SBF較長天數的支架有較佳的骨特異蛋白質表現。總結來說，利用SBF處理後的幾丁聚醣支架具有良好的生物相容性且促進新生骨生成也具有一定的潛力。

Osteoinduction and biocompatibility are important factors in the regeneration of bone tissues. In this study, the chitosan scaffold was modified with the simulated body fluid（SBF）which would create a biomimetic layer on the interface between tissues and scaffolds for the bone formation. The ATR-FTIR analysis showed that the phosphate and calcium ions in the biomimetic layer can generate calcium phosphate deposition and the surface was covered with spheral carbonate-hydroxyapatite.
To investigate the in vivo osteoinduction, the chitosan scaffolds immersed in the SBF for different times（ 3, 5, 7, 14, and 21 days ）were implanted into the calf muscle in male Wistar rats. The tissues blocks containing the scaffolds were harvested at different periods for bone induction and examined histologically. Two histochemical methods, hematoxyline and eosin staining (H & E staining), and alkaline phosphatase staining (ALPase), were performed for the observation of in vivo biocompatibility and early osteoinduction. Immunohistochemical staining of osteopontin (OPN) and bone sialoprotein (BSP) were used to examine osteoblastic phenotypes and to determine possible development stage of osteoinduction in the specimens with SBF modification. The results in this research suggested that the SBF modification would improve the biocompatibility of chitosan scaffolds, revealed by the decrease in foreign body reaction. With the SBF treatment, the expression of osteoblastic differentiation, including ALPase, OPN and BSP, would be also enhanced. Besides, the above tendencies would be more significant with the longer time for SBF immersion. In conclusion, the chitosan scaffolds modified by using SBF bioreactor would possess excellent biocompatibility and high potential in the promotion of bone regeneration.

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