本研究利用立體光刻印刷技術在單層紙張中製造三維微流道，改造市售光固化3D列印機台用於濾紙列印上，並與堆疊式三維紙基微流體晶片進行成效比較，在單層晶片吸收0.5ul溶液時，相較堆疊式晶片有效提升溶液傳遞效率，於各濃度的螢光溶液傳遞中平均有136%的亮度提升。並在單層紙中達到五層分層列印，本晶片開發成功後，能夠應用在更複雜的多流道多層數晶片上。
本晶片利用易取得且成本低廉的纖維素濾紙，使用立體光刻列印技術將樹脂固化在濾紙中，進而分隔製作出具有親水與疏水區域的紙基晶片，並且藉由翻面列印與光罩設計將三維結構微流體通道在單層紙中呈現。
本論文透過四個實驗對製程優化與三個實驗進行晶片製作來測試性能：(1)製作吸光率99.2%之吸光片改造成形平台，避免曝光反射問題，使樹脂固化在0.4秒前呈線性成長；(2)比較染色前後樹脂曝光時間與固化厚度之關係，黑色樹脂在0.01秒的曝光時間控制下可以有4.74 um的高度控制性；(3)利用超聲波清洗未固化樹脂，花費20秒即可將樹脂清除完全；(4)利用後固化製程穩定曝光固化的樹脂性質，透明樹脂約需6分鐘，而黑色樹脂超過30分鐘仍會造成影響，因此判定不適用於單層三維晶片上；(5)製作流道解析度測試光罩，列印薄濾紙流道最小可到60um，厚濾紙則上下底會出現50%左右的失真；(6)比較堆疊式三維晶片與單層三維晶片之溶液傳遞成效，單層吸收0.5ul溶液時，亮度平均提升136%，且標準差較小，因此可判定傳遞較穩定；(7)使用分條式列印，能更好的在單層濾紙建立五層的固化結構。

In this study, Our objective was to establish a systematic method for the creation of three-dimensional microfluidic structures from a single layer of filter paper. Most conventional methods based on the principles of origami or layer-by-layer manufacturing are vulnerable to solvent loss between paper-based flow channels during the flow phase. We sought to overcome this issue through the use of DLP (Digital Light Processing) light curing technology in conjunction with a unique mask design. Briefly, we create a three-dimensional micro flow channel on a single layer of filter paper. When a single-layer wafer absorbs 0.5ul of solution, compared to stacked wafers, the solution transfer efficiency is effectively improved, and there is an average 136% increase in brightness in the transfer of fluorescent solutions of various concentrations. Five-layer printing is achieved in a single-layer paper. It means that this fabrication can be applied to more complex multi-channel and multi-layer chips.
This chip is made by filter paper which is easy to obtain and low-cost. We use stereolithography printing technology to cure resin in the filter paper to separates the hydrophilic and hydrophobic areas. With mask design and flip over printing skill, the three-dimensional structure of the microfluidic channel can be achieved in a single layer of paper.
The conclusions of experiment results are: (1) We add a light-absorbing film on the forming platform to decrease the reflection from the metal surface of the platform; (2) The black resin have the better curing thickness resolution 4.74 um/0.01sec; (3) Using ultrasonic cleaner to clean the uncured resin needs 20 seconds; (4) Using post-curing can stabilize the cured resin not to influence the next exposure. The black resin is not suitable for post-curing because it takes too much time for that; (5) Flow channel size distortion is small on thin filter paper; (5) Single-layer chips have better transfer efficiency and higher uniformity when the amount of solution absorption per unit volume is low; (6) Using batch multi-layer printing, a single-layer filter paper can be built into a five-layer structure.

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