研究生: |
謝坤霖 Kun-Lin Hsieh |
---|---|
論文名稱: |
褐藻膠共聚生物相容性高分子奈米疫苗載體之合成 Synthesis of vaccine nanocarrier of fucoidan hybrid biocompatible polymer |
指導教授: |
蘇舜恭
Shuenn-kung Su |
口試委員: |
陳建光
Jem-Kun Chen 鄭智嘉 Chih-Chia Cheng 許蕙玲 Hui-Ling Hsu |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2017 |
畢業學年度: | 105 |
語文別: | 中文 |
論文頁數: | 124 |
中文關鍵詞: | 明膠 、生物相容性高分子 、褐藻膠 、藥物載體 、化學交聯 、兩親分子 、聚己內酯 |
外文關鍵詞: | Gelatin, Biocompatible polymers, Fucoidan, vaccine carrier, Chemical crosslink, Amphiphilic polymer, Polycaprolactone |
相關次數: | 點閱:273 下載:0 |
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本研究目的是利用褐藻膠(Fucoidan)合成生物相容性疫苗載體,分 為兩個部份,第一部份將天然高分子明膠(Gelatin)經化學交聯 (Chemical crosslinking method) 褐 藻 膠 製 備 天 然 奈 米 粒 子 (Gelatin@Fucoidan nanoparticles,GNFPs)。由 IR 證實氨基交聯結構, 並用電子顯微鏡 SEM 及粒徑分析儀 DLS 分析形貌,得 200~400nm 均 勻粒徑分布。由 UV-vis 測量得 1mg/ml (1FD)組合具有高藥物包覆率 (45%),並且在兩週內僅損失低於 10%藥物量之高穩定性,藉由 CLSM 我們可以觀察到螢光包覆影像。
第二部分則是進行人工高分子聚己內酯與褐藻膠之點擊化學 (Click Reaction)合成褐藻膠及聚己內酯兩親高分子(FD@PCL, Amphiphilic polymer),由 IR 得到疊氮以及炔基的改質訊號,經 NMR 測量褐藻膠與聚己內酯 1:50 比例中,證實三唑環於 8.0ppm 的 1H 訊 號,表示點擊反應成功。在 SEM 觀察油、水相型態轉換,並由 DLS 以及 UV-vis 測得 200nm 之粒徑分佈,與包覆藥物量呈正向關係,具 有高達 86%的包覆率,兩週僅損失 10%蛋白藥物之高穩定性,藉由 CLSM 我們可以觀察兩週後之螢光包覆影像。
In this study, we synthesized biocompatible vaccine nanocarrier hybrid polysaccride Fucoidan. Our research including two parts:
In the first part, Gelaitn was hybrided with Fucoidan by chemical crosslinking method, a mean size was observed by DLS & SEM images, which located between 200~400nm nanoparticle, crosslinking structure has been comfirmed by IR spectrum. A high loading efficiency (45%) and high stability carrier (only 10% of drug were loss during 2 weeks) was prepared, which was measured by UV-vis spectrum. Furthermore, we observed drug loading image by CLSM after stability test.
In the second part, we utilized Click chemistry to combine Fucoidan and Polycaprolactone, which created Fucoidan-graft-Polycaprolactone amphiphilic polymer(FD@PCL), we comfirmed azide and alkynyl group IR signal at 2100cm-1, and then we identified Triazole ring signal around 8.0ppm of 1H NMR, under FD: PCL=1:50 synthesis condition.
Moreover, we produced high stability, high drug loading efficiency carrier transforming from THF to water condition, surprisingly we discovered that size distribution approximately located at 200nm and increased while drug loading, eventually demonstrated 86% encapsulation efficiency, which maintain below 10% loss during one month, measuring by UV-vis spectrum. At last, we observed nanoparticles containing green fluorescence images by CLSM.
Keywords:Gelatin、Biocompatible polymers、Fucoidan、vaccine carrier、 Chemical crosslink、Amphiphilic polymer、Polycaprolactone.
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