微脂粒是由脂質分子分散在水相中所形成的，其結構類似細胞膜。膽固醇在細胞膜中是一重要組成份，因此當要模擬細胞膜時常會加入膽固醇。由於生物體中含有大量的生物型界面活性劑，如膽鹽。若要使用微脂粒來作為藥物載劑，勢必會與界面活性劑產生交互作用，甚至被其所溶解。因此，本文探討膽鹽及添加膽固醇對微脂粒穩定性的影響。
先利用UV吸收光譜探討膽鹽對微脂粒的溶解作用，然後再探討膽固醇對膽鹽溶解微脂粒作用之影響及其在此過程所扮演之角色。藉由嵌入動力學探討膽固醇對微脂粒系統之安定比與臨界混合微胞濃度的影響。另外再觀察膽鹽及膽固醇與微脂粒系統之Re值及K值之間的關係。最後則利用廣角X光繞射來觀察微脂粒與混合微胞之結晶結構變化。

Liposomes form by the dispersion of lipids in the aqueous medium. Their structure is similar to cell membrane. Cholesterol is an important
component of the cell membrane. When one attempts to simulate cell membrane , one often needs to incorporate cholesterol into the liposome formulation. Because of the presence of a large amount of biosurfactants ( for example , bile salt. ) in the organism. Liposomes as drug carriers can interact strongly with the biosurfactant. Liposomes may even be solubilized by biosurfactant. Therefore , the primary goal of this project was to investigate the effect of bile salt ( deoxycholic acid , sodium salt , NaDC ) and cholesterol on the colloidal stability of liposomes.
First , we used UV / visible measurements spectrum to investigate the solubilization of NaDC in the bilayer of liposomes. Then , the effect of cholesterol on the solubilization of NaDC in the bilayer of liposomes and the role of cholesterol in the solubilization process were studied . By the incorporation kinetics experiments , the effects of cholesterol on the stability ratio and the critical mixed micelle concentration of liposome systems were investigated. In addition , correlations between NaDC and cholesterol and Re and K values of liposome systems were established. Finally , WXRS spectra were obtain to study the difference between the crystal structures of liposomes and mixed micelles.

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