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研究生: 張芸瑄
Yun-Xuan Zhang
論文名稱: 牛樟芝萃取物複合敷料於促進傷口癒合之評估
Evaluation of Antrodia cinnamomea Extract Compound Dressing in Promoting Wound Healing
指導教授: 白孟宜
Meng-Yi Bai
口試委員: 鄭詠馨
Yung-Hsin Cheng
葉伊純
Yi-Cheun Yeh
學位類別: 碩士
Master
系所名稱: 應用科技學院 - 醫學工程研究所
Graduate Institute of Biomedical Engineering
論文出版年: 2021
畢業學年度: 109
語文別: 中文
論文頁數: 160
中文關鍵詞: 牛樟芝敷料傷口癒合生物相容性創傷實驗
外文關鍵詞: Antrodia cinnamomea, Dressing, Wound healing, Biocompatibility, Trauma experiment
相關次數: 點閱:255下載:0
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摘要 I Abstract II 目錄 III 圖目錄 V 表目錄 VIII 第1章 緒論 1 1.1 研究動機與目的 1 1.2 實驗設計 2 第2章 文獻回顧 3 2.1 皮膚構造及功能 3 2.1.1 表皮 3 2.1.2 真皮 3 2.1.3 皮下組織 3 2.2 傷口護理 6 2.2.1 傷口修復機制 7 2.2.2 傷口敷料類型 7 2.3 牛樟芝文獻回顧 10 2.3.1 已知活性成分介紹 10 2.3.2 萃取技術 14 2.3.3 牛樟芝現有應用回顧 15 2.3.4 牛樟芝現有傷口敷料應用回顧 16 第3章 材料及方法 18 3.1 藥品及材料 18 3.2 儀器及設備 20 3.3 牛樟芝敷料 22 3.3.1 敷料製備 22 3.3.2 含藥敷料製備 23 3.3.3 掃描式電子顯微鏡分析 24 3.4 細胞實驗 26 3.4.1 細胞選用 26 3.4.2 細胞解凍活化 27 3.4.3 細胞繼代培養 28 3.4.4 細胞計數 29 3.4.5 細胞冷凍保存 30 3.4.6 細胞存活率分析(MTT Assay) 31 3.5 動物創傷實驗(一般傷口模型) 33 3.5.1 實驗動物 33 3.5.2 敷料製備 34 3.5.3 創傷手術 36 3.5.4 傷口影像紀錄與面積估算 37 3.5.5 傷口床再生組織病理切片分析 38 3.5.6 肝腎病理切片藥物毒性分析 39 3.6 動物創傷實驗(糖尿病傷口模型) 40 3.6.1 實驗動物 40 3.6.2 敷料製備 41 3.6.3 糖尿病誘發 43 3.6.4 創傷手術 44 3.6.5 傷口影像紀錄與面積估算 45 3.6.6 傷口床再生組織病理切片分析 46 第4章 結果與討論 47 4.1 牛樟芝敷料 47 4.1.1 掃描式電子顯微鏡分析 47 4.2 體外細胞株之生物相容性測試 48 4.2.1 對3T3細胞存活率分析(Cell Viability) 48 4.2.2 對RAW細胞存活率分析(Cell Viability) 48 4.2.3 對Caco-2細胞存活率分析(Cell Viability) 49 4.2.4 對HepG2細胞存活率分析(Cell Viability) 49 4.3 體內活體動物之創傷實驗(一般傷口模型) 50 4.3.1 小鼠體重紀錄 50 4.3.2 小鼠創傷沾黏指數 51 4.3.3 小鼠傷口面積紀錄 52 4.3.4 小鼠傷口病理切片 54 4.3.5 小鼠肝腎病理切片 55 4.4 體內活體動物之創傷實驗(糖尿病傷口模型) 56 4.4.1 小鼠血糖紀錄 56 4.4.2 小鼠體重紀錄 57 4.4.3 小鼠創傷沾黏指數 58 4.4.4 小鼠傷口面積紀錄 59 4.4.5 小鼠傷口病理切片 61 第5章 結論 62 參考文獻 64

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