β-三鈣磷酸鹽（β-tricalcium phosphate, β-TCP）粉末廣泛應用於
生物墨水中的填料。本研究探討了甘胺酸-硝酸鹽作為形貌修飾劑對
噴霧乾燥法製備的β-TCP 顆粒結構的影響，藉由添加不同重量百分
比例的甘胺酸硝酸鹽（0, 3.5, 10.1 及28.3 wt%）。為了評估結果，通
過將粉末與磷酸鹽緩衝生理鹽水和膠原蛋白粉末混合製備成生物墨
水。隨後，這些生物墨水由3D 列印機列印成複合支架。其中為了
評估顆粒形狀對可成形性的影響，進行了包括相組成、形貌形狀、
比表面積、顆粒大小及體外細胞活性在內的檢驗。首先，微觀結構
顯示，添加甘胺酸硝酸鹽後形成了多孔片狀和固體不規則形狀。其
次，在生物墨水之流變性測試中使用甘胺酸處理之β-TCP 相對於未
添加的展現出較高的黏度。隨後，在成形性評估中，使用甘胺酸處
理之β-TCP 同樣展現出較高的成形性，相比未添加的甘胺酸處理之
β-TCP。此外，生物相容性測試顯示，在不同添加比例的甘胺酸處
理之β-TCP 複合支架， 細胞存活率範圍為139.70%±2.64%至
149.56%±3.50%。本研究強調了甘胺酸作為前驅物在調節顆粒形貌
的關鍵作用，從而在列印過程中提高了支架的可成形性，同時保持
了優異的生物相容性。

Beta-tricalcium phosphate (β-TCP) particles are widely employed as
fillers in bio-ink formulations. This study explored the influence of
glycine precursor nitrate as a morphology modifier on the structure of β-
TCP particles via the spray drying method. The amounts of glycine
precursor nitrate added to the β-TCP precursor solution (0, 3.5, 10.1, and
28.3 wt%) were investigated. To scope out the results, bio-inks were
synthesized by incorporating powder with phosphate-buffered saline and
collagen powder. Subsequently, these bioink formulations were processed
into scaffolds by using a 3D printer. To assess the impact of particle
shapes on formability, comprehensive characterization was conducted
encompassing phase composition, morphology shape, specific surface
area, and particle size. Furthermore, cell viability was assessed through
the MTT assay. Initially, the microstructure result revealed the formation
of porous flakes and solid irregular shapes following the addition of
glycine precursor nitrate. Secondly, for the printability measurements,
bioinks and scaffolds prepared with glycine precursor treated β-TCP
exhibited higher viscosity and formability compared to those prepared
with pure β-TCP. Also, biocompatibility testing demonstrated cell
viability ranging from 139.70%±2.64% to 149.56±3.50% upon the
application of glycine precursor nitrate with β-TCP at the various adding
amounts. This study highlighted the pivotal role of glycine precursor as a
precursor in modulating particle morphology, thereby enhancing scaffold
formability during printing while maintaining excellent biocompatibility.

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