研究生: |
廖顯仁 Xian-ren Liao |
---|---|
論文名稱: |
PSF薄膜接枝DADS對其血液相容性之探討 Effect of diallyl disfulfide grafting on the hemocompatibility of polysulfone membranes |
指導教授: |
楊銘乾
Ming-Chien Yang |
口試委員: |
李振綱
Cheng-Kang Lee 張樂心 Le-Shin Chang 宮輔辰 FU-CHEN KUNG |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2009 |
畢業學年度: | 97 |
語文別: | 中文 |
論文頁數: | 56 |
中文關鍵詞: | 聚碸 、二烯丙基二硫 、活性氧 、膽固醇 、三酸甘油酯 、內毒素 |
外文關鍵詞: | polysulfone, diallyl disulfide, reactive oxygen species (ROS), cholesterol, triglyceride, endotoxin |
相關次數: | 點閱:366 下載:2 |
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本研究在聚碸 (polysulfone, PSF) 薄膜表面上接枝一層二丙烯基二硫化物 (diallyl disulfide, DADS)。所得之薄膜使用接觸角測試與傅里葉轉換紅外光譜儀,探討其表面特性。以凝血時間 (coagulation times, CT) 探討其血液相容性,並使用血漿來進行血小板凝聚的評估,然後進行改質前後的PSF膜抗氧化力與清除膽固醇、三酸甘油酯與內毒素的能力評估。結果顯示,改質後的PSF膜比未改質的PSF膜具有較長的凝血時間並能有效減少血漿蛋白質吸附,且降低自由基的能力更高。此外,DADS接枝於PSF膜上能增加PSF膜的血液相容性。由全部實驗數據顯示,DADS改質後的PSF膜具有消除膽固醇、三酸甘油酯和內毒素的能力。
Diallyl disulfide (DADS) was grafted on the surface of polysulfone (PSF) membranes. The surface was characterized with contact angle measurement and Fourier transform infrared spectrometer (FTIR). Coagulation times (CT) were evaluated in vitro for hemocompatibility and platelet aggregation were evaluated using human blood. The effect of DADS modification on the reduction of oxidative stress, removal of cholesterol, triglyceride and endotoxin were then evaluated. The results showed that the modified PSF membranes exhibited longer CT and less adsorption of plasma proteins than the unmodified PSF membrane. In addition, the CL counts of hydrogen peroxide and superoxide values for modified PSF membranes were more stable than those for unmodified PSF membrane. These results demonstrate that DADS bonding can improve the blood compatibility of PSF membrane. In addition, DADS-modified PSF membranes exhibited ability to remove cholesterol, triglyceride, and endotoxin.
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