表面電漿共振(Surface Plasmon Resonance, SPR)是一種發生在金屬與介電物質介面上的物理現象，被廣泛的應用於生物檢測與免疫組織化學上，可有效的分析檢測物質之微小濃度折射率變化。傳統SPR影像感測器的影像分析是採用五步還原演算法解相，擷取多張不同相位的干涉條紋影像做演算法計算。然而，於擷取影像的過程中，系統容易受到環境因素在光能量擷取上產生誤差，不僅解相步驟較為繁瑣費時，也無法即時監測結果。

本論文提出一具新穎性方法以增強表面電漿共振空間相位生醫感測系統。由於低同調光源干涉具有穩定性與易分辨性，因此我們利用低同調干涉之寬頻光源做為參考基準點，同時並聯雷射光源來做生醫感測，以解決傳統SPR相位感測器無法即時監測與判讀之問題。不同於傳統SPR感測器使用繁瑣的五步還原演算法，此論文運用窗口傅立葉轉換技術解析感測端光場的相位資訊，以檢測不同干涉條紋影像的微小位移變化。另外，本系統使用通訊波段光源，除了使干涉系統具有遠端感測功能外，系統之靈敏度亦較使用可見光波段光源更佳。

於本論文中，我們成功量測固定化Capture DNA前後之相位靈敏度，證明經硫醇修飾與硫基還原反應後的Capture DNA可以鍵結於金膜表面並抓取Target DNA。另外專一性實驗，則證明我們使用之Capture DNA與Target DNA之間具有高度專一性。本架構量測microRNA-21之相位靈敏度為4.62×〖10〗^6 (rad /RIU)，解析度為1.75×〖10〗^(-8) (RIU)，此與理論值相符。

Surface plasmon resonance (SPR) is a physical phenomenon that occurs between the interface of metal and dielectric materials. It has been extensively utilized in bio-detection and immunochemistry for its great efficiency of analyzing the refraction index of detected materials. The typical SPR image sensor is utilizing the five-step algorithm to resolve the sensitivity and resolution through interference fringe images caused various phases. However, in the process of image capture, the system is susceptible to environmental quality and the phase retrieval is more complex.

In this thesis, a novel approach to improve SPR spatial phase biosensing was demonstrated. Because of the stability and resolvability of low coherence light source, we utilized the low coherence light source and laser source for reference and sensing, respectively through the cascaded optical fiber low coherence interferometry (OFLC), which the conventional SPR sensor could not monitor the sensing result in real-time. Unlike the complicated Hariharan five-frame algorithm in the conventional SPR, a windowed Fourier transform was utilized to analyze the phase information from the sensing light field to detect different interference fringes. Moreover, the telecommunication wavelength possessing the remote sensing feature will be taken in OFLCI for better sensitivity than visible light source.

We successfully demonstrated the Capture DNA phase sensitivity before and after immobilization. The Capture DNA could bond with the gold film surface through Thiol-Modified Oligonucleotide Reduction and then capture Target DNA. Moreover, the excellent specificity between Capture DNA and Target DNA is also experimentally illustrated. Finally, the sensitivity and resolution are shown 4.62×〖10〗^6 (rad /RIU) and 1.75×〖10〗^(-8) (RIU), respectively.

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