本論文研究在高分子薄膜上修飾聚乙二醇(poly-ethylene glycol)的簡易方法，以降低蛋白質和細胞在薄膜表面上的吸附。首先藉由多巴胺(dopamine)的自我氧化聚合，而於薄膜表面形成聚多巴胺，此聚多巴胺極易與具有一級胺的分子形成共價結合，因此丙炔胺(propargylamine)能鍵結在聚多巴胺修飾的薄膜上，使得其結構中的炔基暴露在薄膜表面。經由點擊化學(click chemistry)的作用能輕易準確地將含有N3之mPEG接附在薄膜表面。接附在薄膜表面的PEG可由X-ray photoelectron spectroscopy(XPS)確認；其表面型態可用Atomic force microscope(AFM)觀察。表面PEG化之薄膜具有對BSA蛋白質和纖維母細胞(fibroblast)的抗沉積能力；經由Nessler試劑可計算出約有8.0e-02μmole的PEG可被固定於1cm2薄膜表面。

In this study, a facile method was developed to modify the surfaces of polymeric membranes with polyethylene glycol (PEG) to reduce the fouling of proteins and cells. Dopamine was first self-polymerized on membrane surface by oxidation via air exposure. Since the adherent polydopamine is strongly prone to couple with primary amine containing molecules, propargylamine was then coupled to the dopamine treated membrane surface. With alkyane groups exposed, the synthesized PEG-azide was easily grafted to the membrane surface via “click chemistry”. The existence of PEG on the membrane surface was confirmed by X-ray photoelectron spectroscopy; The surface morphology was studied by atomic force microscope. The antifouling capability of the PEGylated membrane was evaluated by incubating with bovine serum albumin solution (BSA) and growing fibroblast on top of it. 8.0e-02μmole PEG can be immobilized on 1cm2 membrane surface via evaluation of Nessler’s reagent.

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