研究生: |
梁晏銘 Yan-ming Liang |
---|---|
論文名稱: |
高壓氧和高壓空氣對軟骨組織工程的影響 The effect of hyperbaric oxygen and air in cartilage tissue engineering |
指導教授: |
洪伯達
Po-Da Hong 戴念梓 Niann-Tzyy Dai |
口試委員: |
高震宇
none |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 醫學工程研究所 Graduate Institute of Biomedical Engineering |
論文出版年: | 2011 |
畢業學年度: | 99 |
語文別: | 中文 |
論文頁數: | 52 |
中文關鍵詞: | 高壓氧 、高壓空氣 、軟骨組織工程 |
外文關鍵詞: | hyperbaric oxygen, hyperbaric air, cartilage tissue engineering |
相關次數: | 點閱:266 下載:0 |
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由於關節軟骨組織修復能力有限,對於罹患關節疾病的病人來言,發展組織工程軟骨是有其必要性。本研究使用脂肪來源之人類成體幹細胞,配合軟骨誘導培養液及明膠(gelatin)/聚己內酯(polycaprolactone)材料,用以探討高壓氣體環境對其組織工程軟骨之影響。實驗結果顯示,雖然各氣體壓力環境刺激的細胞生長速度低於控制組,但高壓氧及高壓空氣對GAGs濃度的濃度提升有明顯幫助。而透過Real-time PCR 分析結果發現2.5ATA air及2 ATA oxygen 環境刺激能夠加速提升軟骨特徵基因-SOX9、aggrecan、collagen II。綜合細胞生長、基質分泌及軟骨基因之表現,在2.5 ATA air 和2 ATA oxygen環境刺激下能幫助軟骨細胞生成及基質合成,具有應用於軟骨缺陷修復之潛力。
Owing to of the limited repair capacity of articular cartilage, it is essential to develop tissue-engineered cartilage for patients suffering from joint disease. Human adipose tissue derived stromal cells (ASCs) were seeded on the gelatin/polycaprolactone biocomposites to study the effects of hyperbaric oxygen and air on cartilage tissue engineering.
The results show the chondrocytes under hyperbaric oxygen and air’s stimulation, in histological analyzes, cartilage matrix was observed in groups and increased over 15 days; glycosaminoglycans syntheses have significant increase comparing to control;chondrogenic-specific gene expression of type II, aggrecan, and SOX9 was increased in until the fifteenth day.
Based on the data of cell proliferation, matrix accumulation and gene expression, 2.5 ATA air group and 2 ATA oxygen group contribute to chondrogenesis and cartilage matrix syntheses, both groups have potential in cartilage regeneration.
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