本論文為開發出一種雷射劃線石墨烯(Laser Scribed Graphene, LSG）技術於製備生物電極，並評估電化學特性於4-硝基苯酚(4-Nitrophenol)量化感測應用。本研究開發的LSG電極是一種由聚醯亞胺膜當源材料，並以CO2雷射燒蝕轉化成圖案化之三維石墨烯材料，其表現有優異的導電性、高比表面積和極佳電化學穩定性。透過雷射功率、劃線燒蝕時間以及溼度等參數調控，我們可精準控制LSG的形貌及優化的石墨烯結構，以實現最佳化對4-硝基苯酚的(4-NP)電化學的感測性能。後續我們更進一步透過電聚合法製備聚二氧乙基噻吩(PEDOT)鍍層以進行LSG之表面修飾，透過循環伏安法(Cyclic Voltammetry, CV)以及微分脈衝伏安法(Differential Pulse Voltammetry, DPV)提升其對4-NP的感測效能。

We development a Laser Scribed Graphene (LSG) technology for the fabrication of biosensors and the evaluation of its electrochemical properties in the quantification of 4-Nitrophenol (4-NP). The LSG electrode developed in this study is a three-dimensional graphene material patterned from a polyimide film using CO2 laser scribing, exhibiting excellent conductivity, high specific surface area, and exceptional electrochemical stability. By adjusting parameters such as laser power, scribing time, and humidity, we can precisely control the morphology of LSG and optimize the graphene structure to achieve the best electrochemical sensing performance for 4-NP. Furthermore, we employed electro-polymerization to prepare a poly(3,4-ethylenedioxythiophene) (PEDOT) coating on the LSG surface, enhancing its sensing efficiency for 4-NP through cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques.

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