本研究之主要目的為開發一微型過濾晶片，可以適用在低壓及高壓的操作環境中，整合於晶片中之濾膜為商用濾膜，擁有成本低廉、易於取得的優點，然而此種濾膜材質為與晶片基材不同，在晶片封裝時屬於異質黏合，製程較為複雜。本研究以PMMA作為晶片材料並提出環形結構晶片設計，配合UV光固化膠黏合製程進行黏合，改善橫向洩漏的問題及提升晶片黏合強度；並以螺旋型流道幾何改善檢體於晶片內流動情況，降低檢體流動不順的現象。由於過濾晶片應用領域廣泛，高壓應用的部分，實驗將以黏度較高的矽油做為檢體進行過濾壓力量測，並對於不同流道尺寸之晶片進行黏合強度測試，並分析其影響；低壓應用則以人類全血作為檢體並分析流量、濾膜孔徑、血細胞比容對於血漿與血細胞的分離效率之影響。

Integration a filter into a microfluidic chip with the benefit from the easily selecting of the pore size due to the availability of the market was approached and reported from various studies. One of the major drawbacks of this approach is the leakage between the filter and the polymeric microfluidic chip/microchannel therefore a novel assembly approach to create a microfluidic chip with embedding the filter for sample separation without the leakage was conducted in this study The microchannel was fabricated on the PMMA substrates and the polycarbonate-based filter was sandwiched between PMMA substrates. The bonding between the PMMA substrates was based on UV glue, the UV glue was injected into a designed ring structure around the filter to prevent the leakage across the edge of the filter. Spiral microchannel geometry was designed to improve the flow situation in the flow channel, reducing ineffective filtration area. Several microfluidic chips with various pore size filters were fabricated for experiments. In order to test the functionality of the fabricated chip, various experiments including the bonding strength test, filtration pressure measurement and blood separation experiment were conducted.

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