Halobacterium salinarum紫膜 (purple membrane, PM) 內部的細菌視紫質(bacteriorhodopsin, BR)受光激發後可產生光電流響應。本論文利用已建立之自動化光電流二維掃描系統對所製備PM晶片的微分光電流響應進行掃描分析與應用測試。研究分為四部分：第一部分以不同聚焦鏡調整雷射光密度，探討入射光單位面積強度對PM晶片光電流響應大小之影響，結果顯示兩者間呈指數關係。第二部分利用此自動化量測系統對以不含或添加有不同尺寸之氧化石墨烯 (grapheme oxide, GO)之架橋所製備之PM晶片以聚焦雷射進行掃描，分析其光電流分佈，結果發現架橋中若添加GO並再以微流清洗後，可使PM晶片光電流分佈更為均勻，顯示GO確實可減少因PM疊層所造成局部較高光電流之出現；第三部分為以聚焦雷射激發並掃描PM晶片以模擬視網膜成像，發現在小範圍中PM晶片光電流之掃描分佈可模糊呈現所預設的影像。最後部分則是以生長有牙菌斑之玻璃片遮蔽PM晶片光路，並進行掃描，結果發現因半透明微生物膜具有三維結構，導致聚焦雷射點被散射而使PM晶片受光面積增加，反而使晶片光電流上升。

The purple membrane (PM) of Halobacterium salinarum contains bacteriorhodopsin (BR), which generates photocurrents upon illumination. This research aimed to analyze the differential photocurrents of PM chips obtained using an automatic scanning system we had previously set up, as well as to study the potential applications of this system. Four topics were investigated. First, by tuning the optical intensity of the illuminating laser with lens of different focal lengths, a power relationship between the differential photocurrent densities and the incident optical intensities was obtained. Second, scanning the coating regions of PM chips led to a finding that the chips prepared with oxidized avidin complexed with graphene oxide of different sizes as the linker had more uniform photocurrent-density distributions than the ones prepared with only pure oxidized avidin. Post-deposition washing the PM chips with a microfluidic flow further improved the uniformity of the distributions, suggesting that GO addition reduced PM stacking, which resulted in locally high photocurrents. Third, blurry images of several different designed graphics were converted from the scanned photocurrent-density profiles of PM chips whose incident light had first passed through a transparency black-printed with each designed graphic, which mimicked retinal imaging. Finally, a slight increase was nevertheless observed in the average of the scanned photocurrent densities of a PM chip whose incident light had first passed through a glass slide covered with grown dental-plaque biofilm. The increase was attributed to the diffusion of the illuminated spot caused by light scattering from the three-dimensional biofilm.

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