對高通量微流體系統的效能來說，快速及均勻的檢體分佈是非常重要的，而檢體分佈的精確性與均勻性和微流道的幾何形狀設計息息相關。此研究的目的為在T字形的微流道中，將液珠(檢體)均勻的分散至各個反應流道，並瞭解幾何形狀設計對液珠均勻分散的影響。使用氣液兩相流的模組進行數值模擬，以觀察液珠要分開時在內部所產生的壓力分佈及流速變化，並完成改良型的設計，使液珠內部的流速變化達到最小，而讓液珠在T字型流道中能較順暢的被分散到反應流道。在此研究中是使用微铣削加工及熱黏合來製作高分子塑材實驗晶片。實驗的方式是將2 μl的檢體用定量分注器注入晶片中，再施以均勻的氣壓驅動檢體分散至各個反應流道中。在分散檢體的過程中，研究的結果證實改良型晶片達到較好的均勻性、較高的可靠度、較少的檢體損失，因此改良型的設計可被應用在設計有多反應流道的高通量微流體晶片。

Rapid and uniform reagent distribution is critical to the performance of a high-throughput microfluidic system, and the geometry design of the microchannel dominations the accuracy and uniformity of the split droplet distribution. This study focuses on the uniform fission of a single liquid droplet in a T-junction microchannel and the aim is to understand the impact of the geometry design to the uniformity of the split droplets. A gas-liquid modeling was realized in the transient numerical simulation to investigate the pressure distribution and the flowing velocities inside the droplet during the splitting process, which leads to a modified design of the T-junction that minimizes the velocity difference inside the droplet and results in a more smooth splitting process. The polymer microfluidic devices were manufactured with micromilling and thermal bonding for droplet distribution experiments. In the multiple experiments, a 2 μl reagent was loaded into the microfluidic device and an uniform pneumatic pressure was applied to push the droplet downward to the T-junction for splitting. The experiment results reveal that a modified T-junction can achieve better uniformity, higher reliability, and less reagent loss in a modified T-junction during the droplet splitting process, which can be applied in designing a high throughput microfluids device for large-scale experiment.

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