有機磷化合物 (Organophosphate compounds) 為一種神經毒素，廣泛用於製造農藥及殺蟲劑，因為在大量使用下對環境或是人體皆造成重大危害，所以在嚴格控管的條件下，能快速的偵測其在環境中的濃度，以及有效處理是一個重要的議題。
本研究所使用的偵測法是以有機磷酸水解酶 (Organophosphorus hydrolase, OPH) 為主，並搭配電化學分析法偵測。因 OPH 具有降解機磷化合物的功能，可應用於有機磷農藥的降解及檢測，是一種具有高發展潛力的酵素。我們藉由基因工程技術來改良 M13 噬菌體 (filamentous bacteriophage) 並以此當作平臺，再利用噬菌體展示技術 (phage display technology) 和亮胺酸拉鍊 (leucine zipper) 將 OPH 酵素展示於噬菌體的pVIII殼蛋白表面上，同時將對低碳鋼 (mild steel) 具有親和性的胜肽鏈也展示於噬菌體的pIII殼蛋白表面上，以利於噬菌體能連結到電極表面。而使用噬菌體固定化酵素催化能力 (kcat/KM = 342.20) 會比未固定化酵素的催化能力 (kcat/KM = 42.66) 高出8.02倍之多，此外偵測的靈敏度也會隨之提高。

Organophosphorus compounds are considered as neurotoxins, and are widely used in the manufacturing of pesticides and insecticides. Due to its extensive usage, it already has affected both human health and environment. Currently, these compounds are regulated and monitored for environmental safety thus, its immediate detection and effective treatment are an important concern.
In this study, the activity of Organophosphorus hydrolase (OPH) was determined via electrochemical analysis and spectrophotometer. Because of its ability to degrade organophosphorus compounds, it can be applied for the detection of organophosphorus compounds. Here, to improve the activity of OPH, the phage display technology (using M13 filamentous bacteriophage) which utilized the leucine zipper as a scaffold was applied. Specifically, the OPH enzyme was displayed on the surface of the pVIII coat protein of M13 phage via leucine zippers. In addition, a mild steel affinity peptide was simultaneously displayed on the pIII coat protein such that the entire phage can be immobilized on the mild steel electrode surface. The most important is that the produced phage can easily recovery by centrifugation rather than labor waste procedure.

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