存在於古生嗜鹽菌Halobacterial salinarum中的紫色細胞膜（purple membrane, PM）是由細菌視紫質（bacteriorhodopsin）和脂質所構成。由於BR受綠光激發後會產生跨PM膜之質子梯度，因此可用此特性來以PM產生光電流及製作生物晶片。本研究探討以核酸適體（aptamer）為生物辨識分子，應用於PM生物光電感測晶片製作。先在胺基化ITO玻璃上塗覆氧化avidin再以生物親和吸附將經biotin修飾的PM固定化，接著再利用化學鍵結法將修飾有胺基的專一性核酸適體固定化於PM晶片上，即可檢測Lactobacillus acidophilus。研究中分析每一層塗覆後PM晶片之光電流響應變化，並用循環伏安法、傅立葉轉換紅外線光譜與拉曼光譜分析以確認核酸適體之固定化，並可吸附微生物。在最適化製程下，檢測L. acidophilus在高濃度時，晶片光電流密度下降90 %左右，檢測靈敏度可低達10 CFU/mL以下；而對同樣高濃度的E. coli菌液進行吸附時，晶片光電流密度下降僅30 %，顯示所製備晶片具有辨識性且專一性佳。

The purple membranes (PM) residing in archeaon Halobacterial salinarum contain bacteriorhodopsin and lipids. A proton gradient across PM is formed when bacteriorhodopsin is activated by illumination with green light; therefore, PM can be readily applied to generating photoelectricity as well as to devising biochips. This research aimed to investigate the employment of aptamers as the recognition element for fabrication of a PM-based photoelectric biosensor chip. The chip was prepared by first bioaffinity-immobilizing biotinylated PM on an aminated ITO electrode with oxidized avidin as the linker, followed by covalently conjugating Lactobacillus acidophilus-specific aminated aptamers on the PM-chip. Photocurrent measurement, cyclic voltammetry (CV), Fourier transform infrared spectroscopy, and Raman spectroscopy were employed to layer-by-layer examine each coating layer, confirming the aptamer immobilization as well as microorganism adsorption. With the optimized chip fabrication process, the aptamer-coated PM chip had a detection limit below 10 CFU/mL for L. acidophilus and the photocurrent generated by the chip declined 90% at high concentration. On the other hand, only 30% photocurrent reduction was observed for the detection of Escherichia coli at the same cell density, suggesting selective recognition of L. acidophilus by the as-prepared aptamer-PM composite chip.

鄭凱如、胡孔政、蔡孟訓、陳秀美“細菌視紫質光敏蛋白於生物感測的新型應用”台灣化學工程學會化工會刊，2011，第58卷，第4期，82-92.
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