研究生: |
田雅汶 Ya-wen Tien |
---|---|
論文名稱: |
製備明膠/聚(氮-異丙基丙烯醯胺)溫感型可控有序多孔性結構薄膜與其藥物釋放之研究 Fabrication of Thermo-Responsive Ordered Porous Film of Gelatin/PNIPAAm and Drug Release Properties |
指導教授: |
陳建光
Jem-Kun Chen |
口試委員: |
邱顯堂
Hsien-Tang Chiu 李俊毅 none 楊銘乾 Ming-Chien Yang |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2013 |
畢業學年度: | 101 |
語文別: | 中文 |
論文頁數: | 91 |
中文關鍵詞: | 明膠 、聚N-異丙基丙烯醯胺 、聚苯乙烯微球 、孔洞結構 、藥物釋放 、抗菌測試 |
外文關鍵詞: | Gelatin, Ploy(N-isopropylacrylamide), Polystyrene microspheres, Porous structure, Drug release, Antibacterial test |
相關次數: | 點閱:391 下載:1 |
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本實驗利用具有溫度敏感性聚(氮-異丙基丙烯醯胺)(Ploy(N-isopropylacrylamide),PNIPAAm)以及具有生物相容性的明膠(gelatin)藉由不同粒徑大小的聚苯乙烯微球(polystyrene , PS)製備出各尺寸的有序多孔性結構薄膜。首先利用N,N'-Methylene bisacrylamide (MBA)將gelatin與PNIPAAm進行交聯後,再使用無乳化劑乳化聚合法聚合出四種粒徑的PS球,接著將四種粒徑大小的PS球加入其溶液中,再將此溶液乾燥成膜並浸泡於甲苯中去除PS球,最後可得四種不同尺寸的gelatin-PNIPAAM多孔性結構薄膜。
利用SEM以及DLS證實本實驗成功製備出不同粒徑大小的PS球,並由FTIR證實gelatin與PNIPAAm交聯,再經由SEM確定gelatin-PNIPAAm多孔性結構薄膜的製備完成,接著使用SEM和CA探討溫度變化對有序多孔性結構薄膜的影響,並在藥物釋放及抗菌測試實驗,證明多孔性結構薄膜在高於LCST的水溶液環境所造成的大孔洞能夠擁有較好的藥物裝載及釋放量,並對於細菌的生長有更好的抑制效果。
In this study, we developed to prepare thermosensitive Ploy(N-isopropylacrylamide)(PNIPAAm) with an biocompatible gelatin and various particle sizes of polystyrene microspheres for the purpose of synthesizing ordered porous film. N,N'-Methylene bisacrylamide (MBA) was used to crosslink gelatin and PNIPAAm. We also used emulsifier-free emulsion polymerization to prepare various particle sizes of monodisperse polystyrene microspheres. The polystyrene microspheres acted as a template to create various size ordered porous film.
Scanning electron microscope(SEM) and dynamic light scattering (DLS) demonstrated various diameters monodisperse polystyrene microspheres were synthesized successfully. The cross-linking reaction of gelatin-PNIAPAAm was confirmed by fourier transform infrared spectroscope(FTIR). And then SEM also revealed the structure of gelatin-PNIPAAm ordered porous film. The thermo-responsive properties of gelatin-PNIPAAm ordered porous film was investigated by SEM and contact angle(CA). Due to the thermo-responsive property of PNIPAAm, the drug release and antibacterial test can confirmed the large pore sizes of the film could have better amounts of drug release and load.
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